1.0 2018-05-07 22:20:36 UTC 2020-05-20 21:04:12 UTC BMDBP00053 BMDBP03121 Enzyme CYP11A1 Secondary metabolites biosynthesis, transport and catabolism A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:11412116). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:11412116). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:11412116). C21-steroid hormone metabolism Lipid metabolism BMDB0000067 Cholesterol BMDB0000068 Epinephrine BMDB0000253 Pregnenolone BMDB0000692 Iron BMDB0001318 4-Methylpentanal BMDB0003178 Heme BMDB0006893 3a,7a-Dihydroxy-5b-cholestane BMDB0000221 NADPH BMDB0001377 Oxygen BMDB0012455 3 alpha,7 alpha,26-Trihydroxy-5beta-cholestane BMDB0000217 NADP BMDB0002111 Water BMDB0006894 3a,7a-Dihydroxy-5b-cholestan-26-al BMDB0000359 3a,7a-Dihydroxycoprostanic acid BMDB0001457 5-b-Cholestane-3a ,7a ,12a-triol BMDB0001231 27-Deoxy-5b-cyprinol BMDB0003533 3a,7a,12a-Trihydroxy-5b-cholestan-26-al BMDB0000601 Coprocholic acid BMDB0002103 27-Hydroxycholesterol BMDB0109593 3beta-Hydroxy-5-cholestenoic acid 21 >1563 bp .AGCAGCAGCTGTGGGGGCAGCATGCTAGCAAGGGGGCTTCCCCTCCGCTCAGCCCTGGT CAAAGCCTGCCCACCCATCCTGAGCACAGTGGGGGAGGGCTGGGGCCACCACAGGGTGGG CACTGGAGAGGGAGCTGGCATCTCCACAAAGACCCCTCGCCCCTACAGTGAGATCCCCTC CCCTGGTGACAATGGCTGGCTTAACCTCTACCATTTCTGGAGGGAGAAGGGCTCACAGAG AATCCACTTTCGCCACATCGAGAACTTCCAGAAGTATGGCCCCATTTACAGGGAGAAGCT TGGCAATTTGGAGTCAGTTTATATCATTCACCCTGAAGACGTGGCCCATCTCTTCAAGTT CGAGGGATCCTACCCAGAGAGATATGACATCCCGCCCTGGCTGGCCTATCACCGATATTA TCAGAAACCCATTGGAGTCCTGTTTAAGAAGTCAGGAACCTGGAAGAAAGACCGGGTGGT CCTGAACACGGAGGTGATGGCTCCAGAGGCAATAAAGAACTTCATCCCACTGCTGAATCC AGTGTCTCAGGACTTCGTCAGCCTCCTGCACAAGCGCATCAAGCAGCAGGGCTCCGGAAA GTTTGTAGGGGACATCAAGGAAGACCTGTTTCACTTTGCCTTTGAGTCCATCACCAATGT CATGTTTGGGGAGCGCCTGGGGATGCTGGAGGAGACAGTGAACCCCGAGGCCCAGAAGTT CATTGATGCCGTCTACAAGATGTTCCACACCAGTGTCCCTCTGCTCAACGTCCCTCCAGA ACTGTACCGTCTATTCAGAACCAAGACTTGGAGGGACCATGTAGCCGCATGGGACACAAT TTTCAATAAAGCTGAAAAATACACTGAGATCTTCTACCAGGACCTGAGACGGAAAACAGA ATTTAGGAATTACCCAGGCATCCTCTACTGCCTCCTGAAAAGTGAGAAGATGCTCTTGGA GGATGTCAAGGCCAATATTACGGAGATGCTGGCAGGGGGTGTGAACACGACATCCATGAC ATTGCAATGGCACTTGTACGAGATGGCACGCAGCCTGAATGTGCAGGAGATGCTGCGGGA GGAGGTTCTGAATGCCCGACGCCAGGCAGAGGGAGACATAAGCAAGATGCTGCAAATGGT CCCACTTCTCAAAGCTAGCATCAAGGAGACGCTGAGACTCCACCCCATCTCCGTGACCCT GCAGAGATACCCTGAAAGTGACTTGGTTCTTCAAGATTACCTGATTCCTGCCAAGACACT GGTGCAAGTGGCCATCTATGCCATGGGCCGAGACCCTGCCTTCTTCTCCAGTCCGGACAA GTTTGACCCAACCAGGTGGCTGAGTAAAGACAAAGACCTCATCCACTTCCGGAACCTGGG CTTTGGCTGGGGAGTGCGGCAGTGCGTGGGCCGGCGGATCGCCGAGCTGGAGATGACCCT CTTCCTCATCCACATTCTGGAGAACTTCAAGGTTGAAATGCAGCATATCGGTGACGTGGA CACCATATTCAACCTCATCCTGACGCCGGACAAGCCCATCTTCCTTGTCTTCCGCCCCTT CAA 520 60333.0 9.15 None >Cholesterol side-chain cleavage enzyme, mitochondrial MLARGLPLRSALVKACPPILSTVGEGWGHHRVGTGEGAGISTKTPRPYSEIPSPGDNGWL NLYHFWREKGSQRIHFRHIENFQKYGPIYREKLGNLESVYIIHPEDVAHLFKFEGSYPER YDIPPWLAYHRYYQKPIGVLFKKSGTWKKDRVVLNTEVMAPEAIKNFIPLLNPVSQDFVS LLHKRIKQQGSGKFVGDIKEDLFHFAFESITNVMFGERLGMLEETVNPEAQKFIDAVYKM FHTSVPLLNVPPELYRLFRTKTWRDHVAAWDTIFNKAEKYTEIFYQDLRRKTEFRNYPGI LYCLLKSEKMLLEDVKANITEMLAGGVNTTSMTLQWHLYEMARSLNVQEMLREEVLNARR QAEGDISKMLQMVPLLKASIKETLRLHPISVTLQRYPESDLVLQDYLIPAKTLVQVAIYA MGRDPAFFSSPDKFDPTRWLSKDKDLIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILE NFKVEMQHIGDVDTIFNLILTPDKPIFLVFRPFNQDPPQA AAA30488.1 P00189 CP11A_BOVIN 1SCC 2ASA 3MZS 3MZS K02130 CYP11A1