1.0 2018-05-07 22:23:26 UTC 2020-05-20 21:04:40 UTC BMDBP00665 Enzyme PAM Involved in copper ion binding Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides (PubMed:2059626). Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (PubMed:2059626). The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (PubMed:2059626). Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (By similarity). BMDB0000119 Glyoxylic acid BMDB0000657 Copper BMDB0001264 Dehydroascorbic acid BMDB0001303 Zinc 7 >2919 bp ATGGCTGGCTTTCGGAGCCTGCTAGTTCTTCTCCTCGTGTTTCCCAGTGGCTGTGTGGGC TTCCGAAGCCCACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGATCATTTTCCAAT GAATGTCTTGGTACCACCAGACCAGTAATTCCTATCGATTCATCAGATTTTGCGTTGGAT ATTCGCATGCCTGGGGTCACACCCAAACAGTCTGATACGTACTTCTGCATGTCAGTGCGT TTGCCAATGGATGAGGAAGCCTTCGTGATTGACTTCAAACCCCGTGCCAGCATGGATACT GTCCATCATATGTTACTGTTTGGATGCAATATGCCAGCGTCCACTGGAAATTACTGGTTT TGTGATGAAGGCACCTGTACAGATAAAGCCAATATTCTCTATGCCTGGGCAAGAAATGCT CCCCCAACGAGACTCCCCAAAGGTGTTGGATTCAGAGTTGGAGGAGAGACTGGAAGCAAA TACTTTGTACTCCAAGTACACTATGGGGATATTAGTGCTTTTAGAGATAATCACAAGGAC TGCTCTGGTGTTTCCTTACACCTCACACGCCTGCCACAGCCTTTAATTGCTGGCATGTAC CTTATGATGTCTGTTGACACTGTTATACCACCAGGAGGGAAAGTGGTGAATTCTGATATT TCATGCCATTATAAAAAGTATCCAATGCATGTCTTTGCCTACAGAGTTCACACTCACCAT TTAGGCAAGGTAGTCAGTGGATACAGAGTAAGAAATGGACAGTGGACCCTGATTGGACGC CAAAGCCCCCAACTGCCACAGGCCTTCTACCCAGTAGAGCACCCAGTGGATGTGAGTTTT GGTGACATCCTGGCAGCAAGATGTGTGTTCACTGGGGAAGGGAGGACAGAAGTCACACAC ATTGGTGGCACGTCTAGTGATGAAATGTGCAACTTATACATTATGTATTACATGGAAGCC AAGCATGCAGTTTCTTTCATGACCTGTACACAGAATGTAGCTCCAGATATATTCAGAACC ATACCACCAGAGGCCAATATTCCAATTCCTGTGAAATCCGATATGGTTATGATGCATGGA CATCACAAAGAAACAGAGAACAAAGACAAGACTTCTTTACTACAGCAGCCCAAACGAGAA GAAGAAGGAGTGTTAGAACAGGGTGATTTCTATTCACTACTTTCCAAGCTGCTAGGAGAA AGGGAAGATGTTGTTCATGTGCATAAATATAATCCTACAGAAAAGGCAGAATCAGAGTCT GACCTGGTAGCTGAGATTGCAAACGTAGTCCAAAAGAAGGATCTTGGTCGGTCCGATACC AGAGAGAGTGCAGAACAGGAGAGGGGCAATGCTATTCTTGTCAGAGACAGAATTCACAAA TTCCACAGACTAGTGTCTACCTTGAGGCCTGCAGAGAGCAGAGTTCTGTCGTTACAGCAG CCCCTACCTGGTGAAGGCACCTGGGAACCAGAACACACAGGAGATTTCCATGTAGAAGAG GCACTGGATTGGCCTGGAGTATACTTGTTACCAGGCCAGGTTTCTGGGGTGGCTCTGGAC CCTCAGAATAATCTCGTGATTTTCCACCGAGGTGACCACGTCTGGGATGGAAACTCTTTT GATAGCAAGTTTGTTTACCAGCAAAGAGGTCTTGGACCAATTGAAGAAGACACTATTCTT GTCATAGATCCAAATAATGCTGCAGTACTCCAGTCCAGTGGAAAAAATCTGTTTTACTTG CCACATGGCTTGAGTATAGATAAAGATGGAAATTATTGGGTCACAGACGTGGCGCTTCAT CAGGTGTTCAAACTAGATCCAAAGAGTAAAGAAGGCCCTCTGCTAACCCTGGGAAGGAGC ATGCAACCAGGCAGTGACCAGAATCACTTCTGTCAGCCCACCGATGTGGCTGTGGATCCA GACACCGGAACCATCTATGTGTCAGATGGCTACTGCAACAGTCGCCTTGTGCAGTTTTCA CCAAGTGGAAAATTCATCACACAGTGGGGAGAAGCGTCTCTAGAGAGCAGTCCTAAACCA GGCCAGTTCAGAGTTCCTCACAGCTTGGCCCTGGTGCCTCCCCTGGGCCAGCTGTGTGTG GCAGACCGGGAAAACGGTCGGATCCAGTGTTTCAAAACCGACACCAAAGAATTTGTGCGC GAGATTAAGCACCCATCGTTTGGAAGAAATGTGTTTGCAATTTCATACATACCAGGTTTG CTCTTTGCGGTGAATGGAAAGCCTTACTTTGAGGACCAAGAACCCGTGCAAGGATTTGTG ATGAACTTTTCCAGCGGGGAAATTATCGATGTCTTCAAGCCAGTGCGCAAGCACTTCGAC ATGCCCCACGACATTGCTGCGTCCGAGGACGGGACCGTGTATGTCGGAGACGCTCACACC AACACCGTGTGGAAGTTCACCTCGACCGAAAAAATGGAACATCGATCAGTTAAGAAGGCT GGCATTGAGGTTCAGGAAATCAAAGAATCCGAGGCAGTTGTTGAAACCAAAATGGAGAAC AAGCCCGCCTCCTCAGAATTGCAGAAGATACAAGAGAAACAGAAGCTGGTCAAAGAGCCG GGCTCCGGAGTGCCGGCTGTTCTCATTACAACCCTTCTGGTTATTCCTGTGGTTGTCCTG CTGGCCATTGCCTTATTTATTCGGTGGAAAAAATCAAGGGCCTTTGGAGATTCTGAACGT AAACTTGAGGCCAGTTCGGGAAGAGTTCTGGGAAGACTTAGAGGAAAGGGAGGCGGAGGC CTAAACCTGGGCAACTTCTTCGCCAGCCGGAAGGGCTACAGCCGGAAGGGGTTCGACCGC CTCAGCACGGAAGGAAGCGACCAGGAGAAAGATGAGGACGCAAGCGAGTCCGAAGAAGAG TACTCGGCCCCGCCGCCCGCCCCTGCCCCTTCCTCCTGA 972 108177.0 6.59 874-897 1-20 >Peptidyl-glycine alpha-amidating monooxygenase MAGFRSLLVLLLVFPSGCVGFRSPLSVFKRFKETTRSFSNECLGTTRPVIPIDSSDFALD IRMPGVTPKQSDTYFCMSVRLPMDEEAFVIDFKPRASMDTVHHMLLFGCNMPASTGNYWF CDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKD CSGVSLHLTRLPQPLIAGMYLMMSVDTVIPPGGKVVNSDISCHYKKYPMHVFAYRVHTHH LGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPVDVSFGDILAARCVFTGEGRTEVTH IGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDIFRTIPPEANIPIPVKSDMVMMHG HHKETENKDKTSLLQQPKREEEGVLEQGDFYSLLSKLLGEREDVVHVHKYNPTEKAESES DLVAEIANVVQKKDLGRSDTRESAEQERGNAILVRDRIHKFHRLVSTLRPAESRVLSLQQ PLPGEGTWEPEHTGDFHVEEALDWPGVYLLPGQVSGVALDPQNNLVIFHRGDHVWDGNSF DSKFVYQQRGLGPIEEDTILVIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDVALH QVFKLDPKSKEGPLLTLGRSMQPGSDQNHFCQPTDVAVDPDTGTIYVSDGYCNSRLVQFS PSGKFITQWGEASLESSPKPGQFRVPHSLALVPPLGQLCVADRENGRIQCFKTDTKEFVR EIKHPSFGRNVFAISYIPGLLFAVNGKPYFEDQEPVQGFVMNFSSGEIIDVFKPVRKHFD MPHDIAASEDGTVYVGDAHTNTVWKFTSTEKMEHRSVKKAGIEVQEIKESEAVVETKMEN KPASSELQKIQEKQKLVKEPGSGVPAVLITTLLVIPVVVLLAIALFIRWKKSRAFGDSER KLEASSGRVLGRLRGKGGGGLNLGNFFASRKGYSRKGFDRLSTEGSDQEKDEDASESEEE YSAPPPAPAPSS AAA30683.1 P10731 AMD_BOVIN 1SDW M18683 PAM