| Identification |
|---|
| BMDB Protein ID
| BMDBP00014 |
| Secondary Accession Numbers
| |
| Name
| Lanosterol 14-alpha demethylase |
| Synonyms
|
Not Available
|
| Gene Name
| CYP51A1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Secondary metabolites biosynthesis, transport and catabolism |
| Specific Function
| A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). |
| Pathways
|
- Steroid Biosynthesis
- Zymosterol biosynthesis
- Zymosterol from lanosterol: step 1/6
|
| Reactions
|
| Lanosterin + 3 Oxygen + 3 NADPH → 4,4-Dimethylcholesta-8,14,24-trienol + Formic acid +3 NADP +4 Water |
details
|
| 24-Hydroxycholesterol + NADPH + Oxygen → (24R)-Cholest-5-ene-3-beta,7-alpha,24-triol + NADP + Water |
details
|
|
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 4 |
| Locus
| Not Available |
| SNPs
| CYP51A1 |
| Gene Sequence
|
>1509 bp
ATGCTGGACTTACTGCAGGCGGGCGGGTCAGTGCTGGGGCAGGCGATGGAGCAAGTAACG
GGCGGCAACCTCGCATCCATGCTGCTCATCGCCTGCGCCTTCACGCTTAGTCTGGTCTAC
CTGTTCCGCCTCGCGGTTGGCCACCTGGCCCCACCACTGCCGACTGGGGCGAAAAGTCCA
CCATACATTGTCTCTCCAATTCCATTCCTTGGACATGCTATAGCATTTGGGAAAAGTCCA
ATTGAATTCCTAGAAGATGCATATGAGAAGTATGGACCTGTATTTAGTTTTACCATGGTG
GGAAAGACGTTTACCTACCTGCTGGGGAGTGAGGCTGCTGCACTGCTTTTTAATAGTAAA
AATGAAGACTTGAATGCCGAGGAAGTCTACAGTCGTCTGACGACACCTGTGTTTGGGAAG
GGAGTTGCCTATGATGTGCCTAATACAGTTTTCTTGGAGCAGAAGAAAATGTTGAAAAGT
GGTCTTAACATAGCCCACTTTAGACAGCACGTGTCTATAATTGAAAAAGAAACAAAAGAA
TACTTTAAAAGTTGGGGAGAAAGTGGAGAAAAAAATTTGTTTGAAGCTCTTTCTGAGCTC
ATCATTTTAACAGCCAGCCATTGTTTACATGGAAAGGAAATCAGAAGTCAACTCAACGAG
AAGGTGGCACAACTGTATGCGGATTTGGATGGAGGTTTTAGCCATGCAGCCTGGCTTTTG
CCAGGCTGGCTCCCTCTGCCTAGTTTCAGACGCAGGGACAGAGCTCATCGGGAGATCAAG
AATATTTTCTACAAGGCAATCCAGAAACGCAGAGAGTCAGGAGAAAAAATTGATGACATT
CTCCAAACTTTACTAGAGTCTACTTACAAGGATGGGCGTCCTCTGACGGATGATGAAGTA
GCAGGCATGCTTATTGGACTGCTCTTGGCAGGGCAGCATACTTCTTCAACTACCAGTGCC
TGGATGGGCTTCTTTTTGGCCAGAGACAAAACACTTCAAGAAAAATGTTTCTTAGAACAG
AAAACAGTCTGTGGTGAGAATCTGCCTCCTCTGACTTATGACCAGCTCAAGGATCTCAAT
TTACTTGATCGCTGTATAAAAGAAACATTAAGACTTCGACCTCCTATAATGACCATGATG
AGACTGGCCAAAACTCCTCTGACTGTGGCAGGGTACACCATTCCTCCAGGACACCAGGTG
TGTGTGTCTCCTACTGTCAATCAGAGACTTAAAGACTCATGGGTAGAGCGTCTGGACTTC
AATCCTGACCGCTACTTAGAGGACAGCCCAGCATCAGGAGAGAAGTTTGCTTACGTGCCA
TTTGGAGCTGGGCGTCATCGTTGCATTGGGGAGAATTTTGCCTATGTTCAAATCAAGACA
ATTTGGTCCACTATGCTTCGTTTATATGAATTTGATCTCATTGATGGGTATTTTCCCACT
GTGAATTATACAACTATGATTCACACCCCTGAAAAACCCATCATCAGATACAAACGAAGA
TCAAAATGA
|
| Protein Properties |
|---|
| Number of Residues
| 502 |
| Molecular Weight
| 56596.0 |
| Theoretical pI
| 8.25 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Lanosterol 14-alpha demethylase
MLDLLQAGGSVLGQAMEQVTGGNLASMLLIACAFTLSLVYLFRLAVGHLAPPLPTGAKSP
PYIVSPIPFLGHAIAFGKSPIEFLEDAYEKYGPVFSFTMVGKTFTYLLGSEAAALLFNSK
NEDLNAEEVYSRLTTPVFGKGVAYDVPNTVFLEQKKMLKSGLNIAHFRQHVSIIEKETKE
YFKSWGESGEKNLFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLL
PGWLPLPSFRRRDRAHREIKNIFYKAIQKRRESGEKIDDILQTLLESTYKDGRPLTDDEV
AGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCFLEQKTVCGENLPPLTYDQLKDLN
LLDRCIKETLRLRPPIMTMMRLAKTPLTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDF
NPDRYLEDSPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPT
VNYTTMIHTPEKPIIRYKRRSK
|
| External Links |
|---|
| GenBank ID Protein
| AAY82452.1 |
| UniProtKB/Swiss-Prot ID
| Q4PJW3 |
| UniProtKB/Swiss-Prot Entry Name
| CP51A_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| DQ078267 |
| GeneCard ID
| CYP51A1 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
