| Identification |
|---|
| BMDB Protein ID
| BMDBP00178 |
| Secondary Accession Numbers
| None |
| Name
| G protein-coupled receptor kinase 5 |
| Synonyms
|
Not Available
|
| Gene Name
| GRK5 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in ATP binding |
| Specific Function
| Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors (Beta-2 adrenergic receptor), muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro) (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 26 |
| Locus
| Not Available |
| SNPs
| GRK5 |
| Gene Sequence
|
>1773 bp
ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCAGGGAAGGAGGCGGA
GGAAAGCGCAAAGGGAAAAGCAAGAAGTGGAAGGAAATCCTGAAGTTCCCTCACATAAAC
CAGTGTGAAGACCTCCGAAGAACCATAGACCGAGATTACTGCAGTTTATGTGACAAACAG
CCAGTCGGGAGGCTGCTTTTCCGGCAGTTCTGCGAAACCAGACCTGGGCTGGAGTCCTAC
ATTCAGTTCCTGGACTCGGTGGCAGAATATGAAGTTACTCCAGATGAAAAACTGGGAGAA
AAAGGGAAGGAAATTATGACCAAGTACCTCACCCCAAAGTCCCCAGTTTTCATCACCCAA
GTTGGACGGGACCTGGTCTCCCAGACGGAGGAGAAGCTCCTTCAGAAACCCTGCAAAGAA
CTCTTCTCGGCCTGCGTGCAGTCTGTCCACGACTACCTGAGGGGAGAACCCTTCCACGAG
TACCTGGACAGCATGTATTTTGATCGCTTTCTCCAGTGGAAGTGGTTGGAAAGGCAACCG
GTAACCAAAAACACTTTCAGGCAGTACCGAGTACTAGGAAAAGGGGGCTTTGGGGAGGTC
TGTGCCTGCCAGGTTCGGGCCACGGGTAAAATGTACGCCTGCAAACGCTTGGAGAAGAAG
AGGATCAAAAAGAGGAAAGGGGAGTCCATGGCCCTGAATGAGAAGCAGATTCTGGAGAAG
GTCAACAGTCGGTTTGTGGTCAACCTGGCCTACGCCTACGAGACCAAGGATGCCCTGTGT
TTGGTCCTGACCATCATGAACGGCGGGGACCTGAAGTTCCACATTTACAACATGGGGAAC
CCCGGCTTCGAGGAGGAGCGCGCCCTGTTCTACGCGGCAGAGATCCTGTGCGGCCTGGAG
GACCTCCACCATGAGAACATTGTCTACAGAGATTTGAAACCTGAAAATATCCTGTTAGAC
GATTACGGCCACATTAGAATTTCGGACCTGGGCCTGGCCGTGAAGATCCCCGAGGGAGAC
CTGATTCGCGGCCGAGTGGGCACCGTTGGCTACATGGCTCCAGAGGTCCTGAACAACCAG
AGGTATGGCCTGAGCCCCGACTACTGGGGTCTGGGCTGCCTCATCTACGAGATGATCGAG
GGCCAGTCGCCCTTCCGCGGCCGCAAGGAGAAGGTGAAGCGGGAGGAGGTGGACCGCCGG
GTGTTGGAGACAGAGGAGGTGTACTCGCACAAGTTCTCCGAGGAGGCCAAGTCCATCTGC
AAAATGCTGCTCACCAAAGATGCAAAGCAGAGGCTGGGCTGCCAGGAGGAAGGGGCCGCG
GAGGTCAAGAGACACCCCTTCTTCAGGAACATGAATTTCAAGCGCTTAGAGGCCGGGATG
TTGGATCCCCCCTTTGTTCCAGATCCCCGGGCCGTGTACTGCAAGGACGTCTTGGACATC
GAGCAGTTCTCCACCGTGAAGGGCGTCAACCTGGACCACACGGACGACGACTTCTACTCC
AAGTTCTCCACGGGCTCTGTGCCCATCCCGTGGCAAAGCGAGATGATAGAAACAGAGTGC
TTTAAGGAGCTGAACGTGTTCGGACCCCACGGTACCCTCTCACCGGACCTGAACCGAAGC
CACCCTCCGGAACCGCCAAAGAAAGGGCTGCTCCAGAGGCTCTTCAAGCGTCAGCATCAA
AACAATTCCAAGAGTTCACCCAATTCCAAGACCAGTTTTAACCACCACATCAATTCAAAC
CACGTGAGTTCAAACTCCACCGGAAGCAGCTAG
|
| Protein Properties |
|---|
| Number of Residues
| 590 |
| Molecular Weight
| 67889.0 |
| Theoretical pI
| 8.32 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
- 25-45
- 55-75
- 109-129
- 141-162
- 168-186
- 198-218
- 255-273
- 284-304
- 308-328
|
| Protein Sequence
|
>G protein-coupled receptor kinase 5
MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHINQCEDLRRTIDRDYCSLCDKQ
PVGRLLFRQFCETRPGLESYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFITQ
VGRDLVSQTEEKLLQKPCKELFSACVQSVHDYLRGEPFHEYLDSMYFDRFLQWKWLERQP
VTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEK
VNSRFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLE
DLHHENIVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQ
RYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSIC
KMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDI
EQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQSEMIETECFKELNVFGPHGTLSPDLNRS
HPPEPPKKGLLQRLFKRQHQNNSKSSPNSKTSFNHHINSNHVSSNSTGSS
|
| External Links |
|---|
| GenBank ID Protein
| AAA17561.1 |
| UniProtKB/Swiss-Prot ID
| P43249 |
| UniProtKB/Swiss-Prot Entry Name
| GRK5_BOVIN |
| PDB IDs
|
|
| GenBank Gene ID
| U01206 |
| GeneCard ID
| GRK5 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
