| Identification |
|---|
| BMDB Protein ID
| BMDBP00452 |
| Secondary Accession Numbers
| None |
| Name
| Flap endonuclease 1 |
| Synonyms
|
Not Available
|
| Gene Name
| FEN1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Replication, recombination and repair |
| Specific Function
| Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 29 |
| Locus
| Not Available |
| SNPs
| FEN1 |
| Gene Sequence
|
>1143 bp
ATGGGAATTCAAGGACTGGCCAAACTGATCGCTGATGTGGCCCCCAGTGCCATCCGGGAG
AATGACATCAAGAGCTACTTTGGCCGCAAGGTGGCCATTGATGCTTCCATGAGCATTTAT
CAGTTCCTGATAGCTGTTCGCCAAGGCGGGGACGTGCTGCAGAATGAGGAGGGGGAGACC
ACCAGCCACCTGATGGGCATGTTCTACCGCACCATCCGCATGATGGAGAATGGCATCAAG
CCCGTGTACGTCTTTGATGGCAAGCCACCGCAGCTCAAGTCCGGGGAGCTGGCCAAACGC
AGCGAGCGGCGGGCCGAGGCTGAGAAGCAGCTGCAGGAGGCTCAGGCCGCCGGGGCTGAG
GCGGAGGTGGAAAAGTTTACGAAGCGGCTGGTGAAGGTCACCAAGCAACACAATGACGAG
TGCAAGCATCTCCTGAGCCTCATGGGCATCCCGTACCTCGACGCACCCAGTGAGGCGGAG
GCTAGCTGTGCGGCCCTGGTGAAGGCTGGCAAAGTCTACGCCGCGGCCACAGAGGACATG
GATTGCCTGACGTTCGGCAGCCCGGTGCTCATGCGGCACCTGACTGCCAGTGAGGCCAAG
AAGCTGCCCATCCAGGAGTTCCACCTGAGCCGGATCCTGCAGGAGCTGGGCCTCAACCAG
GAGCAGTTTGTGGATCTGTGCATCCTGCTGGGCAGTGACTACTGTGAGAGCATCCGGGGC
ATCGGGCCCAAGCGGGCCATGGACCTCATCCAGAAGCACAAGAGCATCGAGGAGATTGTG
CGGCGGCTGGACCCCAACAAGTACCCCGTGCCGGAAAACTGGCTCCACAAGGAGGCTCAG
CAGCTCTTCCTGGAGCCTGAGGTGCTGGACCCAGAGTCTGTGGAGCTGAAGTGGAGCGAG
CCCAATGAAAAAGAGCTCATCAAGTTCATGTGTGGGGAGAAGCAGTTCTCCGAGGAGCGA
ATCCGCAGTGGGGTCAGGCGGCTGAGCAAGAGCCGCCAGGGCAGCACCCAGGGCCGCCTG
GATGATTTCTTTAAGGTGACCGGCTCCCTCTCTTCCGCCAAGCGCAAGGAGCCAGAGCCC
AAGGGAGCCGCTAAGAAGAAGGCAAAGACTGGGGCAGCAGGGAAGTTCAAAAGAGGAAAA
TAA
|
| Protein Properties |
|---|
| Number of Residues
| 380 |
| Molecular Weight
| 42510.0 |
| Theoretical pI
| 8.74 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Flap endonuclease 1
MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGET
TSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQEAQAAGAE
AEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDM
DCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRG
IGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAQQLFLEPEVLDPESVELKWSE
PNEEELIKFMCGEKQFSEERIRSGVRRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEP
KGAAKKKAKTGAAGKFKRGK
|
| External Links |
|---|
| GenBank ID Protein
| AAX46374.1 |
| UniProtKB/Swiss-Prot ID
| Q58DH8 |
| UniProtKB/Swiss-Prot Entry Name
| FEN1_BOVIN |
| PDB IDs
|
|
| GenBank Gene ID
| BT021527 |
| GeneCard ID
| FEN1 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
