Showing Protein Myosin light chain kinase, smooth muscle (BMDBP00459)
| Identification | |||
|---|---|---|---|
| BMDB Protein ID | BMDBP00459 | ||
| Secondary Accession Numbers | None | ||
| Name | Myosin light chain kinase, smooth muscle | ||
| Synonyms | Not Available | ||
| Gene Name | MYLK | ||
| Protein Type | Enzyme | ||
| Biological Properties | |||
| General Function | Involved in actin binding | ||
| Specific Function | Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells (By similarity). | ||
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| GO Classification | Not Available | ||
| Cellular Location | Not Available | ||
| Gene Properties | |||
| Chromosome Location | 1 | ||
| Locus | Not Available | ||
| SNPs | MYLK | ||
| Gene Sequence |
>3531 bp ATGGATTTCCGCGCCAACCTGCAGCGGCAGGTGAAGCCGAAGACCCTGTCAGAGGAGGAG AGGAAAGTGCATGGCCCCCAGCAGGTGGACTTCCGCTCTGTCCTAGCCAAGAAGGGGACT CCCAAGACCCCTGTGCCTGAGAAGGTACCCCCTCCAAAACCGGCCACGCCAGATTTTCGC TCAGTGTTGGGCAGTAAGAAAAAATTACCAACAGAGAATGGTAGCAATAACACTGAGGCC TTGAATGCCAAGGCAGCAGAAGGTCTCAAGCCTGTGGGCAATGCCCAGCCTTCAGGGTTC CTGAAACCCGTGGGCAACGCCAAGCTGGCTGATACCCCCAAACCCTTGAGCAGCACCAAG CCAGCAGAGACCCCCAAACCCTTGGGTAATGTCAAGCCAGCTGAGACCCCCAAACCCTTG GGCAGCACCAAGCCAGCAGAAACCCCCAAACCCTTGGGCAGCACCAAGCCAGCAGAGACC CCAAAACCCTTGGGTAACGTCAAGCCAGCTGAGACCCCCAAACCCTTGGGTAACATCAAG CCAACTGAGACCCCCAAACCCTTGGGCAGCACCAAGCCAGCAGAAACCCCCAAACCCTTG GGCAGCACCAAGCCAGCAGAGACCCCAAAACCCTTGGGTAATGTCAAGCCAGCCGAGACC CCCAAACCCTTGGGTAATGTCAAGCCAGCTGAGACCCCCAAACCCTTGGGTAATGTCAAG CCAGCTGAGACCCCTAAACCTGTGAGCAATGCCAAGCCAGCCGAAACCCTCAAACCTGTG GGCAACGCCAAGCCAGCTGAGACCCCCAAACCCTTGAGTAACGTCAAGCCAGCTGAAACC CCCAAACTTGTGGGCAATGCCAAGCCAGCTGAGACCTCTAAACCCTTGGATAACGCCAAG CCAGCTGAGGCCCCCAAACCCTTGGGCAATGCCAAGCCGGCTGAAATCCCAAAACCCACT GGGAAAGAAGAACTTAAGAAGGAGATTAAGAATGATGTGAACTGCAAGAAGGGCCATGCA GGGGCCACAGATAGTGAAAAGAGACCAGAGAGCCGAGGGACAGCCCCGACCTTCGAGGAG AAACTACAAGATCTCCATGTGGCAGAGGGTCAGAAGCTGCTGCTCCAGTGCCGGGTGTCC TCTGACCCCCCAGCCACCATCACTTGGACACTGAATGGAAAGACGCTCAAGACCACCAAG TTCATCGTCCTCTCCCAAGAAGGCTCCTTGTGCTCCGTCTCCATCGAGAAGGCACTGCCC GAGGACAGAGGCTTATACAAGTGTGTAGCCAAGAACAGTGCCGGCCAGGCTGAGTCCTCC TGCCAAGTCACCGTGGACGTTCCAGATGCCCCCACCAGTGAAAATGCCAAGGCCCCGGAG ATGAAAGCCCGGAGGCCGAAGAGCTCTCTTCCTCCAGTGCTAGGAACAGAAAGTGACGCA ACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCCATGCCGCCTCAGATC ATCCAGTTTCCTGAGGACCAGAAGGTGCGAGCAGGAGAGTCTGTGGAGCTCTTTGGTAAG GTGGCCGGCACCCAGCCCATCACCTGCACCTGGATGAAGTTCCGAAAGCAGATCCAGGAC AGCGAGCACATCAAGGTGGAGAACAGCGAGCAGGGCAGCAAGCTCACCATCCGGGCGGCA CGCCAGGAGCACTGTGGCTGCTACACCCTGCTGGTGGAGAACAAACTGGGCAGCAGGCAG GCCCAAGTCAACCTCACCGTGGTGGACAAGCCAGACCCCCCAGCCGGTACACCTTGTGCC TCTGACATCCGAAGCTCCTCACTGACCTTGTCCTGGTATGGCTCCTCGTATGATGGGGGC AGCGCCGTACAGTCATACAGTGTCGAGATCTGGGACTCAGTGGACAAGACGTGGAAGGAA CTAGCCACGTGCCGCAGCACTTCTTTCAACGTCCAGGACCTGCTGCCTGACCGAGAATAT AAGTTCCGTGTGCGTGCAATCAATGTCTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAA CTCACAGCCTTGGGAGAGAAACCTGAAGAAGAGCCGAAGGACGAAGTGGAGGTGTCAGAT GATGATGAGAAGGAGCCTGAGGTCGACTACCGGACCGTCACGGTCAATACCGAGCAGAAA GTGTCCGACTTCTACGACATCGAAGAGAGACTAGGATCTGGGAAATTTGGACAGGTCTTT CGACTTGTAGAAAAGAAAACTGGAAAAATCTGGGCAGGGAAATTCTTCAAGGCGTATTCG GCCAAGGAGAAAGAGAACATCCGGCAGGAGATCAGCATCATGAACTGCCTGCACCACCCC AAGCTGGTCCAGTGTGTGGACGCCTTCGAAGAGAAGGCCAACATCGTCATGGTCCTGGAG ATCGTGTCCGGGGGTGAGCTGTTCGAGCGCATCATTGATGAGGACTTTGAGCTGACGGAG CGGGAGTGCATCAAGTACATGAAGCAGATCTCAGAGGGGGTGGAGTACATCCACAAGCAG GGCATCGTCCACCTGGACCTCAAGCCCGAGAACATCATGTGTGTCAACAAGACAGGCACC AGGATCAAGCTCATCGACTTCGGTCTGGCCAGGAGGTTGGAGAATGCGGGGTCTCTGAAG GTCCTCTTTGGCACCCCGGAGTTCGTCGCACCCGAAGTGATCAACTATGAGCCCATTGGC TATGCCACGGACATGTGGAGCATCGGGGTCATCTGCTACATCCTGGTCAGTGGACTGTCC CCCTTCATGGGTGACAATGACAACGAAACCTTAGCCAACGTTACCTCAGCCACCTGGGAC TTTGACGACGAGGCCTTCGACGAGATCTCCGATGACGCCAAGGATTTTATCAGCAACTTG CTGAAGAAGGATATGAAAAACCGCCTGAACTGCACCCAGTGCCTTCAGCATCCATGGCTA ATGAAAGACACCAAGAACATGGAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAAGTAC ATGGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCATCGGAAGACTGTCC TCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAACCAGC CCGCTCAATGCAGAAAAACTAGAATCTGAAGATGTCTCCCAAGCTTTCCTGGAGGCTGTC GCTGAGGAGAAGCCCCATGTAAAACCCTACTTTTCCAAGACCATTCGTGACTTAGAAGTT GTGGAGGGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCGGACCCTGAAGTC GTCTGGTTCAAAGATGACCAATCAATCCGGGAGTCCCGCCACTTCCAGATAGACTACGAT GAGGATGGGAACTGCTCTTTAATCATTAGTGATGTCTGCGGGGATGATGATGCCAAGTAC ACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAGCAGAGCTCATCGTGGAA ACCATGGAGGAAGGAGAAGGAGAAGGGGGAGAAGAGGAGGAGGAAGAGTGA |
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| Protein Properties | |||
| Number of Residues | 1176 | ||
| Molecular Weight | 128825.0 | ||
| Theoretical pI | 6.43 | ||
| Pfam Domain Function | Not Available | ||
| Signals |
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| Protein Sequence |
>Myosin light chain kinase, smooth muscle MDFRANLQRQVKPKTLSEEERKVHGPQQVDFRSVLAKKGTPKTPVPEKVPPPKPATPDFR SVLGSKKKLPTENGSNNTEALNAKAAEGLKPVGNAQPSGFLKPVGNAKLADTPKPLSSTK PAETPKPLGNVKPAETPKPLGSTKPAETPKPLGSTKPAETPKPLGNVKPAETPKPLGNIK PTETPKPLGSTKPAETPKPLGSTKPAETPKPLGNVKPAETPKPLGNVKPAETPKPLGNVK PAETPKPVSNAKPAETLKPVGNAKPAETPKPLSNVKPAETPKLVGNAKPAETSKPLDNAK PAEAPKPLGNAKPAEIPKPTGKEELKKEIKNDVNCKKGHAGATDSEKRPESRGTAPTFEE KLQDLHVAEGQKLLLQCRVSSDPPATITWTLNGKTLKTTKFIVLSQEGSLCSVSIEKALP EDRGLYKCVAKNSAGQAESSCQVTVDVPDAPTSENAKAPEMKARRPKSSLPPVLGTESDA TVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVAGTQPITCTWMKFRKQIQD SEHIKVENSEQGSKLTIRAARQEHCGCYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPCA SDIRSSSLTLSWYGSSYDGGSAVQSYSVEIWDSVDKTWKELATCRSTSFNVQDLLPDREY KFRVRAINVYGTSEPSQESELTALGEKPEEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQK VSDFYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHP KLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMKQISEGVEYIHKQ GIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIG YATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNL LKKDMKNRLNCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLS SMAMISGLSGRKSSTGSPTSPLNAEKLESEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEV VEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKY TCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE |
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| External Links | |||
| GenBank ID Protein | AAB25794.1 | ||
| UniProtKB/Swiss-Prot ID | Q28824 | ||
| UniProtKB/Swiss-Prot Entry Name | MYLK_BOVIN | ||
| PDB IDs | Not Available | ||
| GenBank Gene ID | S57131 | ||
| GeneCard ID | MYLK | ||
| GenAtlas ID | Not Available | ||
| HGNC ID | Not Available | ||
| References | |||
| General References | Not Available | ||