Showing Protein Polyribonucleotide 5'-hydroxyl-kinase Clp1 (BMDBP00460)
| Identification | |
|---|---|
| BMDB Protein ID | BMDBP00460 |
| Secondary Accession Numbers | None |
| Name | Polyribonucleotide 5'-hydroxyl-kinase Clp1 |
| Synonyms | Not Available |
| Gene Name | CLP1 |
| Protein Type | Enzyme |
| Biological Properties | |
| General Function | Involved in ATP binding |
| Specific Function | Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing (By similarity). |
| Pathways | Not Available |
| Reactions | Not Available |
| GO Classification | Not Available |
| Cellular Location | Not Available |
| Gene Properties | |
| Chromosome Location | 15 |
| Locus | Not Available |
| SNPs | CLP1 |
| Gene Sequence |
>1278 bp ATGGGAGAGGAGGCCAATGATGACAAGAAGCCAACAACTAAATTTGAACTGGAGCGGGAG ACAGAGCTTCGCTTTGAGGTGGAGGCGTCGCAGTCAGTTCAGTTGGAGCTGCTGGCTGGC ATGGCAGAAATCTTCGGCACAGAGCTGACCCGAAACAAGAAGTTCACCTTTGATGCCGGC GCCAAGGTGGCTGTCTTCACTTGGCACGGCTGTTCGCTGCAGCTCAGTGGCCGCACCGAG GTGGCTTACGTCTCCAAGGATACCCCAATGTTGCTTTATCTCAACACGCATACGGCCTTG GAGCAGATGCGGAGGCAGGCAGAGAAGGAGGAGGAGCGAGGCCCCCGGGTGATGGTCGTG GGCCCCACGGACGTGGGCAAGTCCACCGTGTGCCGTCTGCTGCTCAACTACGCGGTGCGT TTGGGCCGCCGGCCCACGTACGTGGAGCTGGATGTGGGCCAGGGCTCTGTGTCCATCCCC GGCACCATGGGGGCCCTGTACATTGAGCGGCCAGCGGACGTTGAGGAGGGGTTCTCCATC CAGGCCCCGCTGGTGTACCACTTCGGCTCCACCACGCCTGGCACCAACATCAAGCTTTAT AATAAGATTACATCTCGTTTAGCCGATGTGTTCAACCAGAGGTGTGAAGTGAACCGCAGG GCCTCGGTGAGCGGCTGTGTCATTAACACCTGTGGCTGGGTCAAGGGCTCTGGTTACCAG GCCCTGGTCCATGCAGCCTCAGCCTTTGAGGTGGATGTCGTTGTGGTTCTGGATCAAGAA CGGCTGTACAATGAACTGAAACGGGACCTGCCTCACTTCGTCCGCACGGTGCTGCTCCCC AAGTCGGGGGGTGTGGTGGAGCGCTCCAAGGACTTCCGGCGGGAATGCCGGGATGAGCGC ATCCGTGAGTATTTCTATGGGTTCCGGGGCTGTTTCTATCCCCATGCCTTCAACGTCAAA TTTTCAGATGTGAAAATCTACAAAGTCGGGGCACCCACCATCCCAGACTCTTGCCTGCCT CTGGGCATGTCTCAGGAAGACAATCAGCTCAAGCTGGTGCCTGTTACCCCCGGCCGAGAT ATGGTGCACCACCTGCTGAGCGTCAGCACGGCGGAGGGCACAGAGGAGAACCTCTCCGAG ACCAGCGTGGCGGGCTTCATCGTGGTGACCAGTGTGGACCTGGAGCACCAGGTGTTCACC GTTCTCTCTCCGGCCCCCCGCCCACTGCCCAAGAACTTCCTTCTCATCATGGATATCCGG TTCATGGATCTTAAGTAG |
| Protein Properties | |
| Number of Residues | 425 |
| Molecular Weight | 47630.0 |
| Theoretical pI | 6.6 |
| Pfam Domain Function | Not Available |
| Signals |
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| Transmembrane Regions | Not Available |
| Protein Sequence |
>Polyribonucleotide 5'-hydroxyl-kinase Clp1 MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLAGMAEIFGTELTRNKKFTFDAG AKVAVFTWHGCSLQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVV GPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSI QAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQ ALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDER IREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRD MVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIR FMDLK |
| External Links | |
| GenBank ID Protein | AAI33500.1 |
| UniProtKB/Swiss-Prot ID | A2VE01 |
| UniProtKB/Swiss-Prot Entry Name | CLP1_BOVIN |
| PDB IDs | Not Available |
| GenBank Gene ID | BC133499 |
| GeneCard ID | CLP1 |
| GenAtlas ID | Not Available |
| HGNC ID | Not Available |
| References | |
| General References | Not Available |