| Identification |
|---|
| BMDB Protein ID
| BMDBP00686 |
| Secondary Accession Numbers
| None |
| Name
| Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 |
| Synonyms
|
Not Available
|
| Gene Name
| JMJD6 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in histone demethylase activity (H3-R2 specific) |
| Specific Function
| Dioxygenase that can both act as a arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Regulates RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. Hydroxylates its own N-terminus, which is required for homooligomerization. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which preferentially demethylates asymmetric dimethylation. Demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), including mono-, symmetric di- and asymmetric dimethylated forms, thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. In collaboration with BRD4, interacts with the positive transcription elongation factor b (P-TEFb) complex in its active form to regulate polymerase II promoter-proximal pause release for transcriptional activation of a large cohort of genes. On distal enhancers, so called anti-pause enhancers, demethylates both histone H4R3me2 and the methyl cap of 7SKsnRNA leading to the dismissal of the 7SKsnRNA:HEXIM1 inhibitor complex. After removal of repressive marks, the complex BRD4:JMJD6 attract and retain the P-TEFb complex on chromatin, leading to its activation, promoter-proximal polymerase II pause release, and transcriptional activation. Demethylates other arginine methylated-proteins such as ESR1. Has no histone lysine demethylase activity (By similarity). Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65 (By similarity). Seems to be necessary for the regulation of macrophage cytokine responses (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 19 |
| Locus
| Not Available |
| SNPs
| JMJD6 |
| Gene Sequence
|
>1212 bp
ATGAACCACAAGAGCAAGAAGCGGATCCGTGAGGCCAAGAGGAGTGCGCGGCCGGAGCTG
AAGGACTCGTTGGACTGGACCCGGCACAACTACTTCGAGAGCTTCCCACTGAACCCGGCG
GCCGTGGCGGACAATGTGGAAAGGGCAGACGCTTTACAACTGTCGGTGGAAGAATTTGTG
GAGCGATATGAAAGACCGTACAAGCCCGTGGTTCTGCTGAATGCCCAAGAGGGCTGGTCT
GCGCAGGAGAAGTGGACTCTGGAGCGCCTCAAAAGGAAATACCGGAACCAGAAGTTCAAG
TGTGGCGAGGACAACGACGGGTACTCGGTGAAGATGAAGATGAAATACTACATCGAGTAC
ATGGAGAGCACCCGCGATGACAGCCCCCTTTACATCTTTGACAGCAGCTACGGGGAACAC
CCCAAAAGAAGGAAACTTTTGGAAGACTACAAGGTGCCAAAGTTTTTCACCGATGACCTT
TTCCAGTATGCGGGGGAGAAGCGCAGGCCCCCTTACCGGTGGTTTGTGATGGGGCCACCT
CGGTCCGGAACAGGAATCCACATCGACCCTCTGGGAACCAGTGCCTGGAATGCCTTAGTT
CAGGGCCACAAGCGCTGGTGCCTGTTCCCTACCAGCACGCCTAGGGAGCTCATCAAGGTG
ACCCGAGAAGAAGGAGGGAACCAGCAAGATGAAGCTATTACCTGGTTTAACATCATCTAT
CCCCGGACGCAGCTTCCAACCTGGCCACCCGAATTCAAACCCCTGGAAATCTTACAGAAA
CCAGGAGAGACTGTTTTTGTACCAGGAGGCTGGTGGCACGTTGTCCTTAATCTTGACACC
ACCATTGCCATCACCCAGAACTTTGCCAGCAGTACCAACTTCCCTGTTGTCTGGCACAAG
ACGGTAAGAGGGAGACCAAAGTTGTCAAGGAAATGGTATAGGATCTTGAAGCAGGAGCAC
CCGGAGTTGGCGGTCCTGGCTGACTCCGTTGACCTGCAGGAGTCCACGGGGATCGCCTCC
GACAGCTCCAGTGACTCCTCCAGCTCCTCCAGCTCCAGCTCGTCCGACTCGGACTCAGAG
TGCGAGTCCGGCTCTGAAGGCGAAGGCACAATGCACCGCAGGAAGAAGAGGCGGACGTGC
GGCATGGTGGGGAACGGGGACACCACCTCGCAGGACGACTGCGTGAGCAAAGAGCGCAGC
TCCTCCAGGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 403 |
| Molecular Weight
| 46527.0 |
| Theoretical pI
| 8.99 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
MNHKSKKRIREAKRSARPELKDSLDWTRHNYFESFPLNPAAVADNVERADALQLSVEEFV
ERYERPYKPVVLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEY
MESTRDDSPLYIFDSSYGEHPKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPP
RSGTGIHIDPLGTSAWNALVQGHKRWCLFPTSTPRELIKVTREEGGNQQDEAITWFNIIY
PRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDTTIAITQNFASSTNFPVVWHK
TVRGRPKLSRKWYRILKQEHPELAVLADSVDLQESTGIASDSSSDSSSSSSSSSSDSDSE
CESGSEGEGTMHRRKKRRTCGMVGNGDTTSQDDCVSKERSSSR
|
| External Links |
|---|
| GenBank ID Protein
| AAX46368.1 |
| UniProtKB/Swiss-Prot ID
| Q58DS6 |
| UniProtKB/Swiss-Prot Entry Name
| JMJD6_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BT021521 |
| GeneCard ID
| JMJD6 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
