| Identification |
|---|
| BMDB Protein ID
| BMDBP00844 |
| Secondary Accession Numbers
| None |
| Name
| Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A |
| Synonyms
|
Not Available
|
| Gene Name
| STT3A |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in dolichyl-diphosphooligosaccharide-protein g |
| Specific Function
| Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets (By similarity). STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A (By similarity). |
| Pathways
|
- Protein glycosylation
- Protein modification
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 29 |
| Locus
| Not Available |
| SNPs
| STT3A |
| Gene Sequence
|
>2118 bp
ATGACCAAGCTGGGATTTTTGCGGTTGTCCTATGAGAAGCAGGACACACTTCTGAAGCTT
CTGATTCTGTCGATGGCTGCTGTGTTATCATTCTCCACTCGTCTCTTTGCTGTCTTGAGA
TTTGAAAGTGTCATCCATGAATTTGATCCGTACTTTAATTATCGCACCACCCGGTTCCTG
GCTGAGGAGGGATTTTATAAATTCCATAACTGGTTTGATGACCGGGCCTGGTACCCCTTG
GGACGAATCATTGGAGGAACAATTTACCCAGGCTTAATGATCACTTCTGCTGCAATCTAC
CATGTCCTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGTGTTCCTGGCC
CCTCTCTTCTCTTCCTTCACTACCATCGTCACGTACCACCTTACCAAAGAGCTCAAGGAT
GCAGGAGCTGGGCTTCTTGCTGCCGCCATGATTGCTGTGGTTCCTGGCTATATCTCCCGG
TCTGTGGCTGGCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTGCTTACCTAT
TACATGTGGATCAAAGCAGTCAAGACTGGTTCCATCTACTGGGCAGCAAAGTGTGCCCTT
GCTTACTTCTACATGGTCTCTTCCTGGGGAGGTTATGTGTTCTTGATCAACTTGATCCCT
CTTCATGTACTAGTGCTGATGCTCACGGGACGTTTCTCGCACCGGATCTACGTGGCGTAC
TGTACTGTCTACTGCCTGGGCACCATTCTTTCCATGCAGATCTCCTTTGTGGGTTTCCAG
CCCGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTGTTTGGTCTCTGCCAGATTCAT
GCCTTTGTGGATTATCTACGCAGCAAGTTGAATCCACAGCAATTTGAAGTTCTTTTCCGA
AGTGTCATCTCCCTAGTGGGCTTTGTCCTTCTCACCATTGGAGCTCTCCTCATGCTGACA
GGAAAAATATCTCCCTGGACGGGGCGTTTCTACTCACTGTTGGACCCTTCCTATGCTAAG
AACAACATCCCCATCATCGCCTCCGTGTCTGAGCATCAGCCTACGACCTGGTCCTCGTAC
TATTTCGACCTACAGCTTCTTGTCTTCATGTTTCCAGTTGGTCTCTATTACTGCTTTAGC
AACCTGTCTGATGCCCGGATTTTCATCATCATGTATGGTGTGACCAGCATGTACTTTTCA
GCTGTAATGGTGCGTCTGATGCTGGTGTTGGCGCCTGTTATGTGCATTCTTTCTGGCATT
GGAGTCTCTCAGGTGCTGTCCACTTACATGAAGAATTTGGACATAAGTCGACAAGACAAG
AAGAGCAAGAAACAGCAGGATTCTACTTACCCTATTAAGAATGAAGTGGCAAGTGGCATG
ATACTGGTCATGGCTTTCTTTCTCATTACCTACACCTTCCACTCGACCTGGGTGACCAGT
GAGGCCTACTCTTCTCCCTCCATTGTGCTGTCTGCTCGTGGTGGAGATGGCAGTAGGATC
ATTTTTGATGATTTTCGAGAAGCGTACTATTGGCTTCGTCACAATACTCCAGAGGATGCG
AAGGTCATGTCATGGTGGGATTATGGCTACCAGATTACAGCTATGGCGAATCGGACAATT
TTAGTGGATAATAACACGTGGAATAATACCCATATATCTCGAGTAGGGCAGGCAATGGCA
TCCACAGAAGAAAAAGCCTATGAGATCATGAGGGAGCTTGATGTCAGCTATGTGCTGGTC
ATTTTTGGAGGCCTCACTGGGTATTCTTCAGATGACATCAACAAATTTCTGTGGATGGTC
CGGATTGGAGGGAGCACAGATACAGGGAAACACATCAAGGAGCACGATTATTATACTCCA
ACTGGGGAGTTTCGTGTGGACCGTGAGGGCTCTCCAGTGCTGCTCAACTGCCTTATGTAC
AAGATGTGTTACTACCGATTTGGACAGGTTTACACAGAAGCCAAGCGTCCACTAGGCTAT
GACCGTGTCCGGAATGCTGAGATTGGGAATAAAGACTTCGAGCTTGATGTCCTAGAAGAA
GCATACACCACAGAACATTGGCTGGTCAGGATATACAAGGTGAAGGACCTGGATAATCGA
GGCTTGTCAAGAACATAA
|
| Protein Properties |
|---|
| Number of Residues
| 705 |
| Molecular Weight
| 80656.0 |
| Theoretical pI
| 8.21 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
- 18-38
- 120-138
- 141-158
- 170-189
- 192-206
- 212-228
- 234-259
- 268-287
- 301-321
- 357-379
- 386-402
- 407-428
- 454-473
|
| Protein Sequence
|
>Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFL
AEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLA
PLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTY
YMWIKAVKTGSIYWAAKCALAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAY
CTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEVLFR
SVISLVGFVLLTIGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSY
YFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGI
GVSQVLSTYMKNLDISRQDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTS
EAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTI
LVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMV
RIGGSTDTGKHIKEHDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPLGY
DRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT
|
| External Links |
|---|
| GenBank ID Protein
| AAI05329.1 |
| UniProtKB/Swiss-Prot ID
| Q2KJI2 |
| UniProtKB/Swiss-Prot Entry Name
| STT3A_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC105328 |
| GeneCard ID
| STT3A |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
