| Identification |
|---|
| BMDB Protein ID
| BMDBP00942 |
| Secondary Accession Numbers
| None |
| Name
| Protein argonaute-2 |
| Synonyms
|
Not Available
|
| Gene Name
| AGO2 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in endoribonuclease activity, cleaving siRNA-p |
| Specific Function
| Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 14 |
| Locus
| Not Available |
| SNPs
| AGO2 |
| Gene Sequence
|
>2583 bp
ATGTACTCGGGAGCCGGCCCCGCACTCGCACCTCCTGCTCCGCCACCGCCACCCATCCAA
GGATATGCCTTCAAGCCTCCACCCAGACCCGACTTCGGGACCTCCGGGAGAACAATCAAG
TTACAGGCCAACTTCTTTGAAATGGACATTCCAAAAATTGACATCTATCACTATGAGTTG
GATATCAAGCCAGAGAAATGCCCGAGGAGAGTTAACAGGGAAATAGTGGAACATATGGTT
CAGCACTTTAAAACACAGATCTTTGGGGATCGGAAACCAGTGTTTGACGGAAGGAAGAAT
CTCTACACAGCAATGCCCCTTCCGATCGGGAGGGATAAGGTGGAGCTGGAGGTCACGCTG
CCGGGAGAAGGGAAGGACCGCATCTTCAAGGTGTCCATCAAGTGGGTGTCCTGCGTGAGC
TTACAGGCGTTACACGATGCACTTTCGGGGCGGCTGCCCAGCGTCCCCTTCGAGACGATC
CAGGCCCTGGACGTGGTCATGAGGCATCTGCCGTCCATGAGGTACACCCCCGTGGGCCGC
TCCTTCTTCACGGCATCTGAGGGCTGCTCCAACCCACTGGGCGGGGGCCGAGAGGTGTGG
TTTGGCTTCCATCAGTCCGTCCGGCCTTCTCTCTGGAAGATGATGCTGAATATCGATGTC
TCGGCAACAGCGTTCTATAAGGCACAGCCAGTCATTGAGTTTGTTTGTGAAGTCTTGGAT
TTTAAAAGTATTGAAGAACAACAGAAACCTCTGACAGATTCCCAAAGGGTCAAGTTTACC
AAAGAAATCAAAGGTCTAAAGGTGGAGATAACGCACTGCGGGCAGATGAAGAGGAAGTAC
CGCGTGTGCAACGTGACCCGGAGGCCGGCCAGCCACCAGACGTTCCCGCTGCAGCAGGAG
AGCGGGCAGACGGTGGAGTGCACGGTGGCCCAGTACTTCAAGGACAGGCACAAGCTGGTT
CTGCGCTACCCCCACCTCCCATGTTTACAAGTCGGACAGGAGCAGAAACACACCTACCTT
CCCCTGGAGGTCTGCAACATAGTGGCAGGACAGAGATGCATAAAAAAGCTGACCGACAAT
CAGACCTCAACCATGATCAGAGCGACGGCCAGGTCGGCCCCCGACCGGCAGGAAGAGATT
AGCAAGCTGATGAGAAGTGCCAGTTTCAATACAGATCCATACGTTCGTGAATTTGGAATC
ATGGTGAAAGACGAGATGACAGACGTGACCGGACGGGTCCTCCAGCCGCCCTCCATCCTC
TACGGGGGCAGGAATAAGGCGATTGCCACCCCAGTCCAGGGCGTGTGGGACATGAGGAAC
AAGCAGTTCCACACGGGCATTGAGATCAAGGTGTGGGCCATCGCCTGCTTCGCCCCCCAG
CGCCAGTGCACGGAGGTGCACCTCAAGTCCTTCACGGAGCAGCTCAGAAAGATCTCGAGA
GACGCGGGCATGCCCATCCAGGGCCAGCCATGCTTCTGTAAATATGCCCAGGGGGCGGAC
AGTGTGGAGCCCATGTTCCGGCACTTGAAAAACACGTACGCTGGCCTGCAGCTGGTGGTG
GTCATCCTGCCGGGGAAGACCCCCGTTTATGCCGAGGTCAAGCGTGTGGGAGACACGGTG
CTGGGCATGGCCACGCAGTGTGTGCAGATGAAGAACGTGCAGAGGACCACGCCGCAGACC
CTGTCCAACCTCTGGCTGAAAATCAACGTCAAACTGGGCGGCGTGAACAACATCCTGCTG
CCGCAGGGGAGGCCTCCGGTGTTCCAGCAGCCCGTCATCTTTCTGGGGGCAGATGTCACT
CACCCCCCCGCCGGGGACGGGAAGAAGCCGTCCATTGCTGCCGTCGTGGGCAGCATGGAT
GCCCACCCAAACCGCTACTGTGCCACCGTGCGCGTGCAGCAGCACCGGCAGGAGATCATC
CAGGACCTGGCGGCCATGGTGCGTGAGCTGCTCATCCAGTTCTACAAGTCCACGCGCTTC
AAGCCCACCCGCATCATCTTCTACCGCGACGGCGTCTCCGAGGGGCAGTTCCAGCAGGTT
CTGCACCACGAGTTACTGGCCATCCGTGAGGCGTGCATTAAGCTAGAGAAGGACTACCAG
CCAGGCATCACGTTCATCGTGGTCCAGAAGAGACACCACACACGGCTCTTCTGCACCGAC
AAGAACGAGCGGGTTGGGAAAAGCGGAAACATTCCAGCAGGCACAACCGTGGACACGAAA
ATCACCCACCCAACTGAGTTTGACTTCTACCTGTGTAGTCATGCTGGCATCCAGGGAACA
AGCAGGCCCTCGCACTACCATGTGCTCTGGGATGACAATCGCTTCTCTTCCGACGAGCTG
CAGATCCTCACCTACCAGCTGTGTCACACCTACGTGCGCTGCACACGCTCCGTGTCCATC
CCGGCGCCGGCATACTACGCTCACCTGGTGGCCTTCCGGGCCAGGTACCACCTGGTGGAC
AAGGAGCACGACAGTGCTGAAGGAAGCCATACCTCTGGGCAGAGTAACGGACGAGACCAT
CAAGCGCTGGCGAAGGCGGTGCAGGTCCACCAAGACACGCTGCGCACCATGTACTTTGCT
TGA
|
| Protein Properties |
|---|
| Number of Residues
| 860 |
| Molecular Weight
| 97388.0 |
| Theoretical pI
| 9.59 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Protein argonaute-2
MYSGAGPALAPPAPPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYEL
DIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTL
PGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGR
SFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLD
FKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQE
SGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDN
QTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSIL
YGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISR
DAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTV
LGMATQCVQMKNVQRTTPQTLSNLWLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVT
HPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRF
KPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTD
KNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDEL
QILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDH
QALAKAVQVHQDTLRTMYFA
|
| External Links |
|---|
| GenBank ID Protein
| AAS21301.1 |
| UniProtKB/Swiss-Prot ID
| Q6QME8 |
| UniProtKB/Swiss-Prot Entry Name
| AGO2_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| AY533678 |
| GeneCard ID
| AGO2 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
