| Identification |
|---|
| BMDB Protein ID
| BMDBP01051 |
| Secondary Accession Numbers
| None |
| Name
| DNA topoisomerase |
| Synonyms
|
Not Available
|
| Gene Name
| TOP3A |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Replication, recombination and repair |
| Specific Function
| Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 19 |
| Locus
| Not Available |
| SNPs
| TOP3A |
| Gene Sequence
|
>3009 bp
ATGATATTTCCGGCCGCCCACCGCGCTCTCCGGGGGCTGCCGCGGCCCGGTACTCGGGCC
CTGACTCGCGCCGCCATGGAGGTGGCCCTGCGAGGCGTGCGGAAAATCCTTTGCGTGGCC
GAGAAGAACGACGCGGCCAAGGGCATAGCCGACTTGCTGTCTGGCGGCCGCATGCGGCGG
AGGGAAGGACTTTCAAGATTCAACAAGATCTATGAGTTTGATTATCATCTGTGTGGCCAG
AATGTCACCATGGTAATGACCTCAGTCTCAGGACACTTGCTGGCCCATGATTTCCGGATG
CAGTTTCGCAAATGGCAGAGCTGCAATCCTCTTGTCCTGTTTGAAGCAGAAATTGAAAAG
TACTGCCCAGAGAATTTTGTAGACATCAAGAAAACTCTGGAGCGAGAGGCCCAGCAATGC
CAGGCCCTGGTGATTTGGACCGACTGCGACAGAGAGGGTGAAAACATTGGGTTTGAGGTC
ATCCACGTGTGCAAGGCCGTGAAGCCCAGCCTGCAGGTGCTGCGAGCCCGTTTCTCCGAG
ATCACCACCCGCGCTGTCCGGACGGCATGTGAAAACCTTACGGAGCCCGACCAGAGAGTG
AGCGATGCCGTGGATGTGAGGCAGGAGTTGGACCTGCGGATCGGGGCGGCCTTCACCAGG
TTCCAGACGCTGCGGCTGCAGAAGATCTTCCCCGAGGTGCTGGCAGAGCAGCTTATCAGC
TACGGCAGCTGCCAGTTCCCCACCCTGGGGTTCGTGGTGGAGAGGTTCAAAGCCATTCAG
GCATTTGTGCCAGAAGTCTTCCACAAGATCCGAGTGACTCATGACCACAGGGACGGCGTG
GTAGAGTTCAGCTGGAAAAGGCATCGTCTCTTCAATCACACGGCATGTCTCGTCCTCTAC
CAGATGTGCATGGAGGATCCCAGGGCGATGGTGGTAGAGGTCAGGTCAAAAGCCAAGAGC
AAGTGGAGGCCTCAGGCTCTGGACACTGTGGAGCTGGAGAAGCTGGCATCTCGGAAGTTG
AGGATAAATGCTAAAGAAACCATGAGGATTGCAGAAAAGCTCTACACCCAAGGGTACATC
AGCTATCCTCGAACTGAAACGAACATTTTCCCCAAAGACCTAGACCTGGCGGCACTGGTG
GAGCAACAGACCCCGGATCCACGCTGGGGGGCCTTTGCTCGAAACATTCTAGAGCGCGGT
GGCCCCACCCCACGCAATGGGAATAAGTCTGACCAAGCGCACCCTCCCATCCACCCCACC
AAATACACTGACAGCCTGCAGGGGGATGAGCAGCGACTGTACGAGCTCATCGTCCGCCAC
TTCCTGGCCTGCTGCTCGCAGGATGCCCAGGGCCAGGAGACCACTGTGGAGATCGACATC
GCGCAGGAGCGCTTCGTGGCCCACGGCCTCATGATCCTGGCCCGGAACTATCTGGACGTG
TACCCGTATGATCGCTGGAGTGACAAGACCCTCCCTGTCTATGAGCAAGGCACCTGCTTT
CAGCCCAGCACTGTGGAGATGGTGGACGGGGAGACCAGTCCCCCCCAGCTGCTCACCGAG
GCTGACCTCATTGCCCTCATGGAGAAGCACGGCATTGGTACGGACGCCACGCACGCTGAG
CACATCGAGACCATCAAGGCCCGGATGTACGTCGGGCTCACGCCAGATAAGCGCTTTCTC
CCTGGGCACCTGGGCATGGGGCTCGTGGAAGGCTATGACTCCATGGGCTACGAGATGTCC
AAGCCTGATCTGCGGGCCGAGCTGGAGGCTGACCTGAAGCTCATCTGCGAGGGGAAGAAG
GACAAGCTGGTGGTCCTGCATCAGCAGGTGCAGAAGTACAAGCAGGTGTTCATCGAAGCC
GTGGCCAAAGCCAGGAAGTTGGACGAGGCCTTGTCCCAGTACTTCGGGGCGGCTACAGAG
GTAGCCCCACAGGACGCCCTCCCGGCTGTGCCCGAGCCCATCAGGAAGTGTCCTCAGTGC
AGCCGGGACATGGTTCTTAAGACCAGAAAGAGTGGCGGGTTTTACCTCGGTTGCACGGGC
TTCCCTGAGTGTCGGTCGGCTGTCTGGTTCCCGGACAGCGTGCTGGAGGCCAGCAGGGAT
GTGAGCGTGTGCCCAGTGTGCCAGCCGCACCCAGTTTACAGGTTGAAGTTCAAGTTTAAA
CGAGGCAGCCTGCCCCCAACCATGCCCCTAGAATTTGTCGGCTGCATCGGCGGGTGTGAT
GAGACCCTGAGGGAGATCCTGGCCCTCAGGTTCTCCCAGGGCGCTGCCCGAGTCAGCCAG
CCTGCCAGCCAGACCTCCGGCTACCTGCAGGCTAGCCAGTCCCTGAACAGAATGGACAGT
GGCCAGCAGGGCCAGCCCCGGCCCCCCGTCACCAGGCCCTCGAAGGCTTTGACCAGGACT
CTTCCTCCACCCGCCGTTGAGAGTGAGAGCAACTCGGTGACCTGCAACTGTGGCCAGGAG
GCCCTGCTGCTCACCGTCCGGAAGGAGGGCCCCAACCAGGGCCGCCAGTTCTACAAGTGT
GGCAGTGGTGGCTGCAGCTTCTTTCTATGGGCTGACAGCGGCCACCCAGGAGCCGGGCCT
CCCTCCTCAGCGTCCAGACCCCCAGGCGGCCCCCTGGAACACCCCCCGGGCTCAGGGACT
CTCTTGGGCGGGTCCAGCAGGCCTGGCAGTGGTGGCAGCGGTGGCGCATCCTGCCTGTGT
GGCCAGCCGGCTGTCACGCGCACCGTGCAGAAGGAGGGGCCCAACCAGGGCCGCCAGTTC
CACACGTGTGCCCAGCCCCGTGAGCACCAGTGCGGCTTCTTCCAGTGGGTGGACGAGAAC
GTGGCCCCAGGGACCTTCGGAGGCCCCCTCTGGCCAGGAAGCAGGAGAGGAACCCAGGGG
CTGGAGGCCAGAAGCAAGAGGCCTCGGGCCGGCTCCTCAGACACAGGGTCTGCAACCAAG
AAACCCCGGAAGTGCAGCCTTTGCCACCAGCCTGGACATACCCGTCCCTTCTGTCCTCAG
AACAGATGA
|
| Protein Properties |
|---|
| Number of Residues
| 1002 |
| Molecular Weight
| 111512.0 |
| Theoretical pI
| 8.59 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>DNA topoisomerase
MIFPAAHRALRGLPRPGTRALTRAAMEVALRGVRKILCVAEKNDAAKGIADLLSGGRMRR
REGLSRFNKIYEFDYHLCGQNVTMVMTSVSGHLLAHDFRMQFRKWQSCNPLVLFEAEIEK
YCPENFVDIKKTLEREAQQCQALVIWTDCDREGENIGFEVIHVCKAVKPSLQVLRARFSE
ITTRAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQKIFPEVLAEQLIS
YGSCQFPTLGFVVERFKAIQAFVPEVFHKIRVTHDHRDGVVEFSWKRHRLFNHTACLVLY
QMCMEDPRAMVVEVRSKAKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYI
SYPRTETNIFPKDLDLAALVEQQTPDPRWGAFARNILERGGPTPRNGNKSDQAHPPIHPT
KYTDSLQGDEQRLYELIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDV
YPYDRWSDKTLPVYEQGTCFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAE
HIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKK
DKLVVLHQQVQKYKQVFIEAVAKARKLDEALSQYFGAATEVAPQDALPAVPEPIRKCPQC
SRDMVLKTRKSGGFYLGCTGFPECRSAVWFPDSVLEASRDVSVCPVCQPHPVYRLKFKFK
RGSLPPTMPLEFVGCIGGCDETLREILALRFSQGAARVSQPASQTSGYLQASQSLNRMDS
GQQGQPRPPVTRPSKALTRTLPPPAVESESNSVTCNCGQEALLLTVRKEGPNQGRQFYKC
GSGGCSFFLWADSGHPGAGPPSSASRPPGGPLEHPPGSGTLLGGSSRPGSGGSGGASCLC
GQPAVTRTVQKEGPNQGRQFHTCAQPREHQCGFFQWVDENVAPGTFGGPLWPGSRRGTQG
LEARSKRPRAGSSDTGSATKKPRKCSLCHQPGHTRPFCPQNR
|
| External Links |
|---|
| GenBank ID Protein
| AAI26548.1 |
| UniProtKB/Swiss-Prot ID
| A0JN73 |
| UniProtKB/Swiss-Prot Entry Name
| A0JN73_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC126547 |
| GeneCard ID
| TOP3A |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
