| Identification |
|---|
| BMDB Protein ID
| BMDBP01112 |
| Secondary Accession Numbers
| None |
| Name
| DNA polymerase delta subunit 2 |
| Synonyms
|
Not Available
|
| Gene Name
| POLD2 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Replication, recombination and repair |
| Specific Function
| Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex. As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS as a component of the DNA polymerase zeta complex. Along with POLD3, dramatically increases the efficiency and processivity of DNA synthesis of the DNA polymerase zeta complex compared to the minimal zeta complex, consisting of only REV3L and REV7. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 4 |
| Locus
| Not Available |
| SNPs
| POLD2 |
| Gene Sequence
|
>1410 bp
ATGTTTTCTGAGCAGGCTGCCCAGAGGGCCCACACCCTGCTGTCTCCGCCATCAGCCAGC
AATGCCACTTTTGCCAGGGTGCCTGTGGTGACTTACACCAATTCCTCGCAGCCCTTCCGG
CTGGGAGAGCGCACGTTTAGCCGGCAGTATGCCCACATTTATGCCACCCGTTTCATCCAG
ATGAGGCCCTTCCTGGTGAGCAGGGCCCAGCAGCGCTGGGGCAGTGGAGTCCTAGTGAAG
AAGCTGTGTGAACTGCAGCCCGGGGAGAAGTGCTGTGTGGTGGGGACCCTCTTCAAGGCC
ATGCCACTCCAGCCCTCCATCCTACGGGAGGTCAGCGAAGAGCACAACCTGCTCCCCCAG
CCTCCTCGGAGCAAATACATCCACCCAGATGATGAACTGATCTTGGAAGATGAATTGCAG
CGTATCAAACTGGAGGGCACCATTGATGTGTCAAAGCTGGTCACAGGAACGGTCCTGGCT
GTGCTGGGCTCTGCAGGAGATGATGGAAAGTTCCTGGTGGAGGACCACTGCTTTGCAGGG
CTTGCCCCTCAGAAGCCTGCATGCCCCCTCGACACAGACAGGTTTGTGCTGCTGGTGTCT
GGTCTGGGCCTGGGTGGAGGCGGGGGCGAGAGCCTGCTGGGCACCCAGCTGCTGGTGGAC
GTGGTGACAGGGCAGCTTGGGGATGAAGGGGAGCAGTGCAGTGCCGCCCACGTCTCCCGG
GTCATCCTTGCCGGGAACCTCCTCAGCCACAACACGCAGAGCAGAGACTCCATCAACAAG
GCCAAGTACTTGACCAAGAAAACCCAGGCTGCCAGTGTGGAGGCTGTCAAGATGCTGGAC
GAAATCCTTCTGCAGCTGAGCGCCTCCGTGCCCGTGGATGTGATGCCAGGCGAATTTGAC
CCAACAAACTACACGCTTCCCCAGCAGCCCCTGCACCCCTGCATGTTCCCTCTGGCCACT
GCCTACTCCACGCTCCAGCTGGTCACCAACCCCTACCAGGCCACCATCGATGGAGTCAGA
TTCCTGGGGACGTCGGGACAGAACGTGAGTGACATCTTCCGATACAGCAGCATGGAGGAT
CACTTGGAGATCCTGGAGTGGACGCTGCAAGTCCGGCACATCAGCCCCACAGCCCCTGAC
ACCCTAGGTTGTTACCCTTCCTATAAAACCGACCCATTTATCTTTCCGGAGTGCCCTCAC
GTCTACTTTTGTGGCAACACCCCCAGCTTTGGCTCTAAAATCATCCAAGGCCCCGAAGAC
CAGACAGTGTTGTTGGTGGCTGTCCCAGACTTCAGCACCACCCAGACCGCCTGTCTCGTG
AACCTGGGCAGCCTGGCCTGCCAGCCCATCAGCTTCTCAGGCTTCGGGGCAGAGGATGAA
GACCTGGGAGGCCTGGGTCTGGGTCCCTGA
|
| Protein Properties |
|---|
| Number of Residues
| 469 |
| Molecular Weight
| 50997.0 |
| Theoretical pI
| 5.13 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>DNA polymerase delta subunit 2
MFSEQAAQRAHTLLSPPSASNATFARVPVVTYTNSSQPFRLGERSFSRQYAHIYATRLIQ
MRPFLVSRAQQRWGSGVLVKKLCELQPGEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQ
PPRSKYIHPDDELILEDELQRIKLEGTIDVSKLVTGTVLAVLGSAGDDGKFLVEDHCFAG
LAPQKPACPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSR
VILAGNLLSHNTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFD
PTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMED
HLEILEWTLQVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIQGPED
QTVLLVAVPDFSTTQTACLVNLRSLACQPISFSGFGAEDEDLGGLGLGP
|
| External Links |
|---|
| GenBank ID Protein
| AAC48475.1 |
| UniProtKB/Swiss-Prot ID
| P49004 |
| UniProtKB/Swiss-Prot Entry Name
| DPOD2_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| U21091 |
| GeneCard ID
| POLD2 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
