| Identification |
|---|
| BMDB Protein ID
| BMDBP01126 |
| Secondary Accession Numbers
| None |
| Name
| DNA polymerase delta catalytic subunit |
| Synonyms
|
Not Available
|
| Gene Name
| POLD1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Replication, recombination and repair |
| Specific Function
| As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 18 |
| Locus
| Not Available |
| SNPs
| POLD1 |
| Gene Sequence
|
>3321 bp
ATGGATGGTAAGCGGCGACCAGGCCCGGGGCCTGGGGTGCCCCCAAAGCGGGCCCGTGGG
GGCCTCTGGGATGAGGATGAGGCATACCGGCCCTCGCAGTTCGAGGAGGAGCTGGCGCTG
ATGGAGGAGATGGAAGCAGAGCGCAGGCTGCAGGAGCAGGAGGAGGAGGAGCTGCAGTCG
GCCCTGGAGGCGGCGGACGGGCAATTCTCCCCAACGGCCATAGATGCCCGCTGGCTTCGG
CCCGCCCCGCCCGCCTTGGACCCCCAGATGGAGCCTCTCATCTTCCAGCAGTTGGAGATC
GACCATTACGTGGCCCCAGCGCGGCCCCTGCCTGGGGCGCCCCCGCCATCCCAGGACTCA
GTTCCCATCCTCCGCGCCTTCGGGGTCACCAACGAGGGGGTCTCCGTCTGCTGCCACATC
CATGGCTTTGCACCCTACTTCTACACCCCAGCGCCCCCTGGTTTTGGACCTGAGCACCTG
AGCGAGCTGCAGCGGGAGCTGAGTGCAGCCATCAGCCGGGACCAGCGCGGGGGCAAGGAG
CTCACCGGGCCGGCCGTGCTGGCGGTAGAGCTGTGCTCCCGGGAGAGCATGTTCGGGTAC
CATGGGCACGGCCCCTCCCCGTTTCTGCGTATCACCTTGGCACTGCCCCGCCTCATGGCA
CCTGCCCGCCGCCTCCTGGAGCAGGGCATCCGCCTGGCCGGCCTCGGCACCCCCAGCTTT
GCGCCCTACGAGGCCAACGTTGACTTTGAGATCCGGTTCATGGTGGACACGGACATCGTG
GGCTGCAACTGGCTGGAGCTCCCAGCCGGGAAATACATCCTGAGGCCGGAGGGGAAGGCC
ACTCTGTGTCAGCTGGAGGCCGACGTGCTGTGGTCAGACGTGATCAGCCACCCGCCGGAA
GGAGAGTGGCAGCGAATCGCCCCTCTGCGCGTGCTCAGCTTCGACATCGAGTGCGCTGGT
CGCAAAGGCATCTTCCCTGAGCCCGAGCGGGACCCCGTGATCCAGATCTGCTCACTGGGC
CTGCGCTGGGGCGAGCCGGAGCCCTTCCTGCGCCTGGCGCTCACCCTGCGGCCCTGCGCC
CCCATCCTGGGCGCCAAGGTGCAGAGCTATGAGCGGGAGGAGGACCTGCTCCAGGCCTGG
TCGACCTTCATCCGCATCATGGATCCCGATGTGATCACCGGCTACAATATCCAGAACTTT
GACCTTCCCTACCTCATCTCCCGGGCCCAGACCCTCAAGGTGCCAGGCTTCCCCTTGCTG
GGCCGTGTGATTGGCCTCCGCTCCAACATCCGGGAGTCGTCCTTCCAGTCCAGGCAGACT
GGCCGGCGGGACAGCAAGGTGGTCAGCATGGTGGGCCGCGTGCAGATGGACATGCTGCAG
GTGCTGCTGCGGGAGTACAAGCTCCGGTCCTACACGCTCAATGCCGTGAGCTTCCACTTC
CTGGGCGAGCAGAAGGAGGACGTGCAGCACAGCATCATCACAGACCTGCAGAACGGTAAC
GACCAGACGCGCCGCCGCCTGGCCGTGTACTGCCTCAAGGACGCCTTCCTACCCCTGCGG
CTGCTGGAGCGGCTCATGGTGCTGGTGAACGCCATGGAGATGGCGCGCGTCACCGGCGTG
CCCCTCGGCTACCTGCTCAGCCGCGGCCAGCAGGTCAAGGTCGTGTCCCAGCTGCTGCGA
CAGGCCATGCGCCAGGGGCTGTTGATGCCCGTGGTGAAGACGGAGGGTGGTGAGGACTAT
ACCGGGGCCACGGTCATCGAGCCGCTGAAAGGGTACTACGACGTTCCCATCGCCACCTTG
GACTTCTCCTCGCTGTACCCGTCCATCATGATGGCCCACAACCTGTGCTACACCACACTC
CTGCGGCCCGGGGCCGCCCAGAAACTGGGCCTGACCGAGGATCAGTTCATCAAGACGCCC
ACGGGGGACGAGTTTGTGAAGGCATCGGTGCGGAAGGGGCTGCTCCCCCAGATCCTGGAA
AACCTGCTCAGCGCCCGGAAGAGGGCCAAGGCCGAGCTGGCCAAGGAGACAGACCCCCTA
CGGCGGCAAGTGTTGGACGGGCGCCAGCTGGCGCTGAAAGTGAGTGCTAACTCTGTGTAC
GGCTTCACTGGCGCCCAGGTGGGCAGGCTCCCGTGCCTGGAAATCTCACAGAGTGTCACC
GGGTTCGGGCGCCAGATGATTGAGAAGACAAAGCAGCTTGTGGAGACCAAGTACACGGTG
GAAAACGGCTACAGCACCAGCGCCAAGGTGGTGTATGGTGACACAGACTCGGTCATGTGC
CGCTTTGGCGTCTCATCCGTGGCTGAGGCGATGGCTTTGGGACGGGAGGCTGCAGACTGG
GTGTCCGGCCACTTCCCCTCGCCCATCCGGCTAGAGTTTGAGAAAGTCTACTTCCCCTAC
CTGCTCATCAGCAAGAAGCGTTACGCAGGCCTGCTCTTCTCCTCCCGGCCGGACGCCCAC
GACCGCATGGACTGCAAGGGCCTGGAGGCCGTGCGCAGGGACAACTGCCCCCTGGTGGCC
AACCTCGTCACCGCCTCGCTGCGCCGCCTGCTCATCGACCGAGACCCCTCGGGCGCCGTG
GCTCATGCACAGGACGTCATCTCCGATCTGCTGTGTAATCGCATTGACATCTCGCAGCTG
GTCATTACCAAGGAGCTGACTCGCGCTGCCGCCGATTACGCGGGCAAGCAGGCCCACGTG
GAGCTGGCCGAGAGGATGAGGAAGCGGGACCCCGGGAGCGCGCCCAGCCTGGGCGACCGC
GTCCCCTACGTGATCATCAGCGCTGCCAAGGGCGTGGCCGCCTACATGAAGTCCGAGGAC
CCCCTGTTCGTACTGGAGCACAGCCTGCCCATCGACACGCAGTACTACCTGGAGCAGCAG
CTCGCCAAGCCGCTCCTGCGCATCTTCGAGCCCATCCTGGGCGAGGGCCGTGCCGAGGCT
GTGCTGCTGCGCGGGGACCACACTCGCTGCAAGACGGTGCTCACGGGGAAGGTGGGCGGC
CTCCTGGCCTTCGCCAAACGCCGGAACTGCTGCATCGGCTGCCGCACTGTCCTCAGCCAC
CAGGGAGCCGTGTGCAAGTTCTGCCAGCCCCGGGAGTCAGAGCTGTACCAGAAGGAGGTG
TCCCACCTGAGTGCCCTGGAGGAGCGATTCTCACGCCTGTGGACGCAGTGCCAGCGCTGC
CAGGGCAGCCTGCACGAGGACGTCATCTGCACCAGCCGGGACTGTCCCATCTTCTACATG
CGCAAGAAGGTGCGGAAGGACCTGGAGGACCAGGAGCGGCTGCTGCGGCGCTTTGGACCC
CCCGGCCCAGAGGCTTGGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 1106 |
| Molecular Weight
| 123709.0 |
| Theoretical pI
| 7.64 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>DNA polymerase delta catalytic subunit
MDGKRRPGPGPGVPPKRARGGLWDEDEAYRPSQFEEELALMEEMEAERRLQEQEEEELQS
ALEAADGQFSPTAIDARWLRPAPPALDPQMEPLIFQQLEIDHYVAPARPLPGAPPPSQDS
VPILRAFGVTNEGVSVCCHIHGFAPYFYTPAPPGFGPEHLSELQRELSAAISRDQRGGKE
LTGPAVLAVELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGIRLAGLGTPSF
APYEANVDFEIRFMVDTDIVGCNWLELPAGKYILRPEGKATLCQLEADVLWSDVISHPPE
GEWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCA
PILGAKVQSYEREEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVPGFPLL
GRVIGLRSNIRESSFQSRQTGRRDSKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHF
LGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAFLPLRLLERLMVLVNAMEMARVTGV
PLGYLLSRGQQVKVVSQLLRQAMRQGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATL
DFSSLYPSIMMAHNLCYTTLLRPGAAQKLGLTEDQFIKTPTGDEFVKASVRKGLLPQILE
NLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGRLPCLEISQSVT
GFGRQMIEKTKQLVETKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADW
VSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVA
NLVTASLRRLLIDRDPSGAVAHAQDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHV
ELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQ
LAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSH
QGAVCKFCQPRESELYQKEVSHLSALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYM
RKKVRKDLEDQERLLRRFGPPGPEAW
|
| External Links |
|---|
| GenBank ID Protein
| AAA30493.1 |
| UniProtKB/Swiss-Prot ID
| P28339 |
| UniProtKB/Swiss-Prot Entry Name
| DPOD1_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| M80395 |
| GeneCard ID
| POLD1 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
