| Identification |
|---|
| BMDB Protein ID
| BMDBP01546 |
| Secondary Accession Numbers
| None |
| Name
| Cholesterol side-chain cleavage enzyme, mitochondrial |
| Synonyms
|
Not Available
|
| Gene Name
| CYP11A1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Secondary metabolites biosynthesis, transport and catabolism |
| Specific Function
| A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). |
| Pathways
|
- C21-steroid hormone metabolism
- Lipid metabolism
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 21 |
| Locus
| Not Available |
| SNPs
| CYP11A1 |
| Gene Sequence
|
>1563 bp
ATGCTAGCAAGGGGGCTTCCCCTCCGCTCAGCCCTGGTCAAAGCCTGCCCACCCATCCTG
AGCACAGTGGGGGAGGGCTGGGGCCACCACAGGGTGGGCACTGGAGAGGGAGCTGGCATC
TCCACAAAGACCCCTCGCCCCTACAGTGAGATCCCCTCCCCTGGTGACAATGGCTGGCTT
AACCTCTACCATTTCTGGAGGGAGAAGGGCTCACAGAGAATCCACTTTCGCCACATCGAG
AACTTCCAGAAGTATGGCCCCATTTACAGGGAGAAGCTTGGCAATTTGGAGTCAGTTTAT
ATCATTCACCCTGAAGACGTGGCCCATCTCTTCAAGTTCGAGGGATCCTACCCAGAGAGA
TATGACATCCCGCCCTGGCTGGCCTATCACCGATATTATCAGAAACCCATTGGAGTCCTG
TTTAAGAAGTCAGGAACCTGGAAGAAAGACCGGGTGGTCCTGAACACGGAGGTGATGGCT
CCAGAGGCAATAAAGAACTTCATCCCACTGCTGAATCCAGTGTCTCAGGACTTCGTCAGC
CTCCTGCACAAGCGCATCAAGCAGCAGGGCTCCGGAAAGTTTGTAGGGGACATCAAGGAA
GACCTGTTTCACTTTGCCTTTGAGTCCATCACCAATGTCATGTTTGGGGAGCGCCTGGGG
ATGCTGGAGGAGACAGTGAACCCCGAGGCCCAGAAGTTCATTGATGCCGTCTACAAGATG
TTCCACACCAGTGTCCCTCTGCTCAACGTCCCTCCAGAACTGTACCGTCTATTCAGAACC
AAGACTTGGAGGGACCATGTAGCCGCATGGGACACAATTTTCAATAAAGCTGAAAAATAC
ACTGAGATCTTCTACCAGGACCTGAGACGGAAAACAGAATTTAGGAATTACCCAGGCATC
CTCTACTGCCTCCTGAAAAGTGAGAAGATGCTCTTGGAGGATGTCAAGGCCAATATTACG
GAGATGCTGGCAGGGGGTGTGGACACGACATCCATGACATTGCAATGGCACTTGTACGAG
ATGGCACGCAGCCTGAATGTGCAGGAGATGCTGCGGGAGGAGGTTCTGAATGCCCGACGC
CAGGCAGAGGGAGACATAAGCAAGATGCTGCAAATGGTCCCACTTCTCAAAGCTAGCATC
AAGGAGACGCTGAGACTCCACCCCATCTCCGTGACCCTGCAGAGATACCCTGAAAGTGAC
TTGGTTCTTCAAGATTACCTGATTCCTGCCAAGACACTGGTGCAAGTGGCCATCTATGCC
ATGGGCCGAGACCCTGCCTTCTTCTCCAGTCCGGACAAGTTTGACCCAACCAGGTGGCTG
AGTAAAGACAAAGACCTCATCCACTTCCGGAACCTGGGCTTTGGCTGGGGAGTGCGGCAG
TGCGTGGGCCGGCGGATCGCCGAGCTGGAGATGACCCTCTTCCTCATCCACATTCTGGAG
AACTTCAAGGTTGAAATGCAGCATATCGGTGACGTGGACACCATATTCAACCTCATCCTG
ACGCCGGACAAGCCCATCTTCCTTGTCTTCCGCCCCTTCAACCAGGACCCGCCCCAGGCG
TGA
|
| Protein Properties |
|---|
| Number of Residues
| 520 |
| Molecular Weight
| 60334.0 |
| Theoretical pI
| 9.01 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
- 30-56
- 77-98
- 113-133
- 140-158
- 178-200
- 229-246
- 288-307
- 319-338
- 350-372
|
| Protein Sequence
|
>Cytochrome P450, family 11, subfamily A, polypeptide 1
MLARGLPLRSALVKACPPILSTVGEGWGHHRVGTGEGAGISTKTPRPYSEIPSPGDNGWL
NLYHFWREKGSQRIHFRHIENFQKYGPIYREKLGNLESVYIIHPEDVAHLFKFEGSYPER
YDIPPWLAYHRYYQKPIGVLFKKSGTWKKDRVVLNTEVMAPEAIKNFIPLLNPVSQDFVS
LLHKRIKQQGSGKFVGDIKEDLFHFAFESITNVMFGERLGMLEETVNPEAQKFIDAVYKM
FHTSVPLLNVPPELYRLFRTKTWRDHVAAWDTIFNKAEKYTEIFYQDLRRKTEFRNYPGI
LYCLLKSEKMLLEDVKANITEMLAGGVDTTSMTLQWHLYEMARSLNVQEMLREEVLNARR
QAEGDISKMLQMVPLLKASIKETLRLHPISVTLQRYPESDLVLQDYLIPAKTLVQVAIYA
MGRDPAFFSSPDKFDPTRWLSKDKDLIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILE
NFKVEMQHIGDVDTIFNLILTPDKPIFLVFRPFNQDPPQA
|
| External Links |
|---|
| GenBank ID Protein
| AAI33390.1 |
| UniProtKB/Swiss-Prot ID
| A3KMX9 |
| UniProtKB/Swiss-Prot Entry Name
| A3KMX9_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC133389 |
| GeneCard ID
| CYP11A1 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
