| Identification |
|---|
| BMDB Protein ID
| BMDBP02204 |
| Secondary Accession Numbers
| None |
| Name
| Target of rapamycin complex subunit LST8 |
| Synonyms
|
Not Available
|
| Gene Name
| MLST8 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657' (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 25 |
| Locus
| Not Available |
| SNPs
| MLST8 |
| Gene Sequence
|
>1059 bp
ATGAATACGTCTCCAGGCACAGTGGGCAGTGACCCCGTCATCTTGGCCACTGCAGGCTAT
GACCACACGGTGCGGTTCTGGCAGGCCCACAGCGGGATCTGTACGCGAACTGTGCAGCAC
CAGGACTCCCAGGTGAACGCGCTGGAGATCACACCCGACCGCACCATGATTGCAGCTGCA
GGTTACCAGCACATTCGCATGTATGATCTCAACTCCAATAACCCCAACCCCATCATCAGC
TACGACGGGGTCAACAAGAACGTCGCGTCGGTGGGCTTCCACGAGGACGGGCGCTGGATG
TACACGGGCGGGGAGGACTGCACAGCCCGGATCTGGGACCTCAGGTCCCGGAACCTGCAG
TGTCAGCGAATCTTCCAGGTGAATGCACCCATTAACTGCGTGTGCCTGCACCCCAACCAG
GCGGAGCTCATCGTGGGTGACCAGAGCGGCGCCATCCACATCTGGGACTTGAAAACTGAC
CACAATGAGCAGCTGATCCCGGAGCCGGAAGTCTCCATCACATCCGCCCACATCGACCCA
GATGCCAGCTACATGGCTGCCGTCAATAGTACTGGGAACTGCTATGTCTGGAACCTGACT
GGAGGCATTGGCGACGAGGTGACACAGCTCATCCCCAAGACCAAGATCCCGGCGCACACC
CGCTATGCCCTGCAGTGCCGCTTCAGCCCCGACTCCACGCTCCTCGCCACCTGCTCGGCC
GACCAGACGTGCAAGATCTGGAGGACGTCCAACTTCTCCCTGATGACAGAGCTGAGCATC
AAGAGCAGTAATCCCGGAGAGTCATCTCGGGGCTGGATGTGGGGCTGCGCCTTCTCAGGA
GACTCGCAATACATCGTCACCGCTTCCTCTGACAACCTGGCCCGGCTCTGGTGTGTGGAG
ACAGGCGAGATCAAGAGAGAGTATGGCGGCCACCAGAAAGCTGTTGTGTGCTTGGCCTTC
ACGACAGCGTGCTGGGCTAGCCTCGTTCCTGGGCACTGCAGGCAGCCGTCCAGGTGGCAC
CAGCCGGGTGTGTCTGTCCAGCTGCCCAGGTCAGCGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 326 |
| Molecular Weight
| 35920.0 |
| Theoretical pI
| 5.72 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Target of rapamycin complex subunit LST8
MNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEITPDRTMIAAA
GYQHIRMYDLNSNNPNPIISYDGVNKNVASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQ
CQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDP
DASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSA
DQTCKIWRTSNFSLMTELSIKSSNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVE
TGEIKREYGGHQKAVVCLAFNDSVLG
|
| External Links |
|---|
| GenBank ID Protein
| AAX46409.1 |
| UniProtKB/Swiss-Prot ID
| Q17QU5 |
| UniProtKB/Swiss-Prot Entry Name
| LST8_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BT021562 |
| GeneCard ID
| MLST8 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
