| Identification |
|---|
| BMDB Protein ID
| BMDBP02651 |
| Secondary Accession Numbers
| None |
| Name
| Mu-type opioid receptor |
| Synonyms
|
Not Available
|
| Gene Name
| OPRM1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in mu-opioid receptor activity |
| Specific Function
| Receptor for endogenous opioids such as beta-endorphin and endomorphin. Receptor for natural and synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone (PubMed:10581406). Agonist binding to the receptor induces coupling to an inactive GDP-bound heterotrimeric G-protein complex and subsequent exchange of GDP for GTP in the G-protein alpha subunit leading to dissociation of the G-protein complex with the free GTP-bound G-protein alpha and the G-protein beta-gamma dimer activating downstream cellular effectors. The agonist- and cell type-specific activity is predominantly coupled to pertussis toxin-sensitive G(i) and G(o) G alpha proteins, GNAI1, GNAI2, GNAI3 and GNAO1, and to a lesser extent to pertussis toxin-insensitive G alpha proteins GNAZ and GNA15. They mediate an array of downstream cellular responses, including inhibition of adenylate cyclase activity and both N-type and L-type calcium channels, activation of inward rectifying potassium channels, mitogen-activated protein kinase (MAPK), phospholipase C (PLC), phosphoinositide/protein kinase (PKC), phosphoinositide 3-kinase (PI3K) and regulation of NF-kappa-B. Also couples to adenylate cyclase stimulatory G alpha proteins. The selective temporal coupling to G-proteins and subsequent signaling can be regulated by RGSZ proteins, such as RGS9, RGS17 and RGS4. Phosphorylation by members of the GPRK subfamily of Ser/Thr protein kinases and association with beta-arrestins is involved in short-term receptor desensitization. Beta-arrestins associate with the GPRK-phosphorylated receptor and uncouple it from the G-protein thus terminating signal transduction. The phosphorylated receptor is internalized through endocytosis via clathrin-coated pits which involves beta-arrestins. The activation of the ERK pathway occurs either in a G-protein-dependent or a beta-arrestin-dependent manner and is regulated by agonist-specific receptor phosphorylation. Acts as a class A G-protein coupled receptor (GPCR) which dissociates from beta-arrestin at or near the plasma membrane and undergoes rapid recycling. Receptor down-regulation pathways are varying with the agonist and occur dependent or independent of G-protein coupling. Endogenous ligands induce rapid desensitization, endocytosis and recycling. Heterooligomerization with other GPCRs can modulate agonist binding, signaling and trafficking properties. Involved in neurogenesis (By similarity). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 9 |
| Locus
| Not Available |
| SNPs
| OPRM1 |
| Gene Sequence
|
>1206 bp
ATGGACAGCGGCGCCGTCCCCACGAACGCCAGCAACTGCACTGATCCCTTCACACACCCT
TCAAGTTGCTCCCCAGCACCTAGTCCCAGCTCCTGGGTCAACTTCTCCCACTTAGAAGGC
AACCTGTCCGACCCATGCGGTCCGAACCGCACCGAGCTGGGAGGGAGCGACAGACTGTGC
CCTTCGGCCGGCAGCCCTTCCATGATCACGGCCATCATCATCATGGCCCTCTACTCCATC
GTGTGCGTGGTGGGGCTCTTCGGAAACTTCCTGGTCATGTATGTGATTGTCAGGTACACC
AAAATGAAGACTGCCACCAACATCTATATTTTCAACCTCGCCCTGGCAGATGCCCTGGCA
ACCAGTACCCTGCCTTTCCAGAGTGTCAATTACCTGATGGGAACATGGCCGTTTGGAACC
ATCCTGTGCAAGATTGTGATCTCCATAGATTACTACAATATGTTCACCAGCATATTCACC
CTCTGCACCATGAGTGTGGATCGCTACATTGCAGTCTGCCATCCTGTCAAGGCCCTGGAT
TTACGCACTCCCCGTAATGCCAAGATCATCAACATCTGCAACTGGATCCTCTCTTCAGCC
ATTGGTCTGCCTGTGATGTTCATGGCAACGACAAAGTACCGGCAAGGTTCCATAGATTGT
ACACTAACATTCTCTCACCCAACGTGGTACTGGGAAAACCTGCTGAAAATCTGTGTTTTC
ATCTTTGCCTTCATCATGCCTATCCTCATCATTACAGTGTGTTATGGGCTGATGATCTTA
CGCCTCAAGAGTGTCCGCATGCTCTCTGGCTCCAAAGAAAAGGACAGGAACCTGCGAAGA
ATCACCAGGATGGTGCTGGTGGTTGTGGCTGTGTTCATTGTCTGCTGGACGCCCATTCAC
ATCTACGTCATCATTAAAGCCTTGATCACAATCCCGGAAACTACTTTCCAGACCGTTTCC
TGGCACTTCTGCATTGCTCTAGGTTATACCAACAGTTGCCTCAACCCCGTCCTTTATGCA
TTTCTGGATGAAAACTTCAAACGATGCTTCAGAGAGTTCTGTATCCCAACTTCCTCCACC
ATTGAGCAGCAAAACTCCACTCGAATTCGTCAGAACACCAGAGACCACCCCTCCACAGCC
AATACGGTGGATAGGACTAACCATCAGCTAGAAAATCTGGAAGCAGAAACCACTCCGTTA
CCCTAA
|
| Protein Properties |
|---|
| Number of Residues
| 401 |
| Molecular Weight
| 45028.0 |
| Theoretical pI
| 8.3 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
- 70-94
- 108-132
- 144-166
- 187-208
- 232-256
- 281-307
- 316-339
|
| Protein Sequence
|
>Mu-type opioid receptor
MDSGAVPTNASNCTDPFTHPSSCSPAPSPSSWVNFSHLEGNLSDPCGPNRTELGGSDRLC
PSAGSPSMITAIIIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALA
TSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALD
LRTPRNAKIINICNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVF
IFAFIMPILIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIH
IYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSST
IEQQNSTRIRQNTRDHPSTANTVDRTNHQLENLEAETTPLP
|
| External Links |
|---|
| GenBank ID Protein
| AAB49477.2 |
| UniProtKB/Swiss-Prot ID
| P79350 |
| UniProtKB/Swiss-Prot Entry Name
| OPRM_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| U89677 |
| GeneCard ID
| OPRM1 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
