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| Identification |
|---|
| BMDB Protein ID
| BMDBP00040 |
| Secondary Accession Numbers
| |
| Name
| Steroid 17-alpha-hydroxylase/17,20 lyase |
| Synonyms
|
Not Available
|
| Gene Name
| CYP17A1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Secondary metabolites biosynthesis, transport and catabolism |
| Specific Function
| A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). |
| Pathways
|
- Lipid metabolism
- Steroid Biosynthesis
|
| Reactions
|
| 21-Deoxycortisol + Water + Acceptor → 11b-Hydroxyprogesterone + Oxygen + Reduced acceptor |
details
|
| Progesterone + Oxygen + Reduced acceptor → 17-Hydroxyprogesterone + Water + Acceptor |
details
|
| 17-Hydroxyprogesterone → Androstenedione |
details
|
| 17a-Hydroxypregnenolone → Dehydroepiandrosterone |
details
|
|
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 26 |
| Locus
| 26q22-q31 |
| SNPs
| CYP17A1 |
| Gene Sequence
|
>1530 bp
GACTGCTGCCACCCAGACACAATGTGGCTGCTCCTGGCTGTCTTTCTGCTCACCCTCGCC
TATTTATTTTGGCCCAAGACCAAGCACTCTGGTGCCAAGTACCCCAGGAGCCTCCCATCC
CTGCCCCTGGTGGGCAGCCTGCCGTTCCTCCCCAGACGTGGCCAGCAGCACAAGAACTTC
TTCAAGCTGCAGGAAAAATATGGCCCCATCTATTCCTTTCGTTTGGGTTCCAAGACGACT
GTGATGATTGGACACCACCAGTTGGCCAGGGAGGTGCTTCTCAAGAAGGGCAAGGAATTC
TCTGGGCGTCCCAAAGTGGCCACTCTAGACATCCTGTCAGACAACCAAAAGGGCATTGCC
TTTGCCGACCATGGTGCCCACTGGCAGCTGCATCGGAAGCTGGCACTGAATGCCTTTGCC
CTGTTCAAGGATGGCAACCTGAAGTTAGAGAAGATCATTAATCAGGAAGCCAATGTGCTC
TGTGATTTCCTGGCCACCCAGCATGGAGAGGCCATAGATCTGTCCGAGCCTCTCTCTCTG
GCGGTGACCAACATAATCAGCTTTATCTGCTTCAACTTCTCCTTCAAGAATGAGGATCCT
GCCCTGAAGGCCATACAAAATGTCAATGATGGCATCCTGGAGGTTCTGAGCAAGGAAGTT
CTGTTAGACATATTCCCTGTGCTGAAGATTTTCCCCAGCAAAGCCATGGAAAAGATGAAG
GGTTGTGTTCAAACGCGAAATGAATTGCTGAATGAAATCCTTGAAAAATGTCAGGAGAAC
TTCAGCAGTGATTCCATCACTAACTTGCTGCACATACTGATCCAAGCCAAGGTGAATGCA
GACAATAACAATGCTGGCCCAGACCAGGATTCAAAGCTGCTTTCAAATAGACACATGCTC
GCTACTATAGGGGACATCTTCGGGGCTGGTGTGGAGACCACCACGTCTGTGATAAAGTGG
ATCGTGGCCTACCTGCTACACCATCCTTCGTTGAAGAAGAGGATCCAGGATGACATTGAC
CAGATTATAGGTTTCAATCGCACCCCAACCATCAGTGACCGGAACCGCCTTGTCCTGCTG
GAGGCGACCATCAGAGAAGTGCTCCGAATCCGGCCTGTGGCCCCTACGCTGATCCCCCAC
AAGGCTGTCATTGACTCCAGCATTGGCGACCTTACCATTGACAAAGGCACAGACGTTGTG
GTCAACCTGTGGGCACTGCATCACAGTGAGAAGGAGTGGCAGCATCCCGACCTGTTCATG
CCCGAGCGCTTCTTGGACCCCACGGGGACGCAACTCATCTCGCCATCATTAAGCTACTTG
CCCTTTGGAGCAGGACCCCGCTCCTGCGTAGGTGAGATGCTAGCCCGCCAGGAGCTCTTC
CTCTTCATGTCCCGGCTGCTGCAGAGGTTCAACCTGGAGATCCCGGATGATGGGAAGCTA
CCTTCTCTGGAGGGCCATGCCAGTCTCGTCTTGCAGATCAAACCTTTCAAGGTGAAGATC
GAGGTGCGCCAGGCCTGGAAGGAAGCCCAG
|
| Protein Properties |
|---|
| Number of Residues
| 509 |
| Molecular Weight
| 57244.0 |
| Theoretical pI
| 8.9 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Steroid 17-alpha-hydroxylase/17,20 lyase
MWLLLAVFLLTLAYLFWPKTKHSGAKYPRSLPSLPLVGSLPFLPRRGQQHKNFFKLQEKY
GPIYSFRLGSKTTVMIGHHQLAREVLLKKGKEFSGRPKVATLDILSDNQKGIAFADHGAH
WQLHRKLALNAFALFKDGNLKLEKIINQEANVLCDFLATQHGEAIDLSEPLSLAVTNIIS
FICFNFSFKNEDPALKAIQNVNDGILEVLSKEVLLDIFPVLKIFPSKAMEKMKGCVQTRN
ELLNEILEKCQENFSSDSITNLLHILIQAKVNADNNNAGPDQDSKLLSNRHMLATIGDIF
GAGVETTTSVIKWIVAYLLHHPSLKKRIQDDIDQIIGFNRTPTISDRNRLVLLEATIREV
LRIRPVAPTLIPHKAVIDSSIGDLTIDKGTDVVVNLWALHHSEKEWQHPDLFMPERFLDP
TGTQLISPSLSYLPFGAGPRSCVGEMLARQELFLFMSRLLQRFNLEIPDDGKLPSLEGHA
SLVLQIKPFKVKIEVRQAWKEAQAEGSTP
|
| External Links |
|---|
| GenBank ID Protein
| AAA30485.1 |
| UniProtKB/Swiss-Prot ID
| P05185 |
| UniProtKB/Swiss-Prot Entry Name
| CP17A_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| M12547 |
| GeneCard ID
| CYP17A1 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
