| Identification |
|---|
| BMDB Protein ID
| BMDBP00783 |
| Secondary Accession Numbers
| |
| Name
| Cytosolic phospholipase A2 |
| Synonyms
|
Not Available
|
| Gene Name
| PLA2G4A |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in calcium ion binding |
| Specific Function
| Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (By similarity). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway. In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific. Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides. Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (By similarity). |
| Pathways
|
Not Available
|
| Reactions
|
| PC(14:0/20:4(5Z,8Z,11Z,14Z)) + Water → Arachidonic acid + LysoPC(14:0) |
details
|
| PE(16:0/16:0) → LysoPE(16:0/0:0) |
details
|
|
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 16 |
| Locus
| Not Available |
| SNPs
| PLA2G4A |
| Gene Sequence
|
>2250 bp
ATGTCATTTATAGATCCTTATCAACACATTATAGTGGAGCACCATTATTCCCACAAGTTC
ACAGTAGTGGTGCTGCGTGCCACAAAAGTGACAAAGGGAACCTTTGGTGACATGCTTGAC
ACTCCAGACCCCTATGTGGAACTTTTCATCTCTTCAACCCCCGACAGCAGGAAGAGAACA
AGACACTTCAATAATGACATAAACCCTGTGTGGAATGAGACCTTTGAATTTATTTTGGAT
CCTAATCAGGAAAATATTTTGGAGATCACTTTAATGGATGCCAATTATGTTATGGATGAA
ACTCTTGGGACGACAACGTTTCCCATATCTTCTATGAAAGTGGGAGAGAAGAAACAGGTT
CCTTTTATTTTCAATCAAGTCACTGAAATGATTCTGGAAATGTCTCTTGAAGTTTGTTCA
TCCCCAGACCTCCGGTTTAGTATGGCTCTGTGTGACCAGGAGAAAGCTTTCAGACAACAG
AGAAAGGAAAACATAAAGGAAAACATGAAGAAACTGCTGGGTCCAAAGAATAGTGAAGGC
TTGCATTCTACACGTGATGTGCCTGTGGTGGCCATACTGGGCTCAGGTGGGGGATTTCGA
GCCATGGTAGGATTCTCTGGTGTGATGAAGGCACTGTATGAGTCAGGGATTTTGGACTGT
GCTACCTACATTGCTGGTCTTTCTGGATCCACATGGTATATGTCGACCTTATATTCTCAC
CCTGATTTTCCAGAGAAAGGGCCAGAGGAGATTAACAAAGAGCTAATGAAAAATGTCAGC
CACAACCCTCTTTTACTTCTCACACCACAGAAAATTAAAAGATATGTTGAGTCTTTATGG
AGGAAAAAAAGTTCTGGACAACCTGTCACCTTTACTGATATCTTCGGGATGTTAATAGGA
GAAACACTAATTCACAATAGAATGAACACTACCCTGAGTAGTTTGAAGGAAAAAGTCAAT
ACTGGGCAGTGTCCTCTGCCGCTTTTCACCTGTCTCCATGTCAAACCCGATGTCTCAGAG
CTGATGTTTGCAGATTGGGTTGAATTTAGTCCATTTGAGATTGGCATGGCTAAATATGGT
ACTTTTATGGCGCCTGACTTATTTGGAAGCAAATTTTTTATGGGAACAGTTGTAAAGAAA
TACGAAGAAAACCCCTTGCATTTCTTAATGGGTGTCTGGGGTAGTGCCTTTTCTATATTG
TTCAACAGAGTCTTGGGAGTTTCTGGATCACAAAGTAAAGGTTCCACAATGGAAGAAGAA
CTAGAAAATATTACAGCAAAGCATATTGTGAGTAATGATAGCTCAGACAGTGATGACGAA
TCACAAGGACCCAAAGGCACTGAACATGAAGAAGCAGAAAGGGAATACCAAAATGATAAT
CAAGCAAGTTGGGTCCAACGTATGCTCATGGCCTTGGTGAGTGACTCAGCTTTGTTCAAT
ACCAGAGAAGGACGTGCCGGGAAGGTGCACAACTTCATGCTGGGCTTGAATCTCAATACA
TCCTATCCAATGTCTCCTTTGAGAGACTTTACCATGCAAGAGTCCCTGGATGAAGACGAA
TTGGATGCCGCTGTCGCAGATCCTGATGAATTTGAGCAGATATATGAGCCTTTGGATGTC
AAAAGTAAAAAGATTCATGTGGTGGACAGTGGCCTCACATTTAACCTTCCATACCCTTTG
ATCCTGAGACCTCAGAGGGGAGTTGATCTCATCATCTCCTTTGACTTTTCTGCAAGACCA
GGTGACTCCAGTCCTCCGTTCAAGGAACTTCTACTTGCAGAAAAGTGGGCTAAAATGAAC
AAGCTCCCCTTTCCAAAGATTGATCCTTACGTATTCGATCGAGAAGGACTGAAGGAATGC
TATGTTTTTAAACCCAAGAATCCTGATGTCGAGAAAGATTGCCCGACTATCATTCACTTT
GTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCTCCAGGTGTTCCAAGAGAAACTAAT
GAAGAGAAAGAAATAGCTGATTTTGATATTTTTGATGACCCTGAATCACCGTTTTCGACC
TTCAACTTTCAATATCCCAATCAAGCATTTAAAAGGCTGCATGATCTCATGTACTTCAAT
ACCCTGAACAACATTGATGTAATAAAGAATGCTATAGTCGAAAGCATCGAATATAGAAGG
CAGAATCCGTCTCGTTGCTCTGTTTCCCTCAGTAGTGTTGAGGCAAGAAGATTCTTCAAC
AAGGAGTTTCTAAGCAAACCCACAGCATAG
|
| Protein Properties |
|---|
| Number of Residues
| 749 |
| Molecular Weight
| 85350.0 |
| Theoretical pI
| 5.18 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
- 12-31
- 67-88
- 100-117
- 137-158
- 170-198
- 204-222
|
| Protein Sequence
|
>Cytosolic phospholipase A2
MSFIDPYQHIIVEHHYSHKFTVVVLRATKVTKGTFGDMLDTPDPYVELFISSTPDSRKRT
RHFNNDINPVWNETFEFILDPNQENILEITLMDANYVMDETLGTTTFPISSMKVGEKKQV
PFIFNQVTEMILEMSLEVCSSPDLRFSMALCDQEKAFRQQRKENIKENMKKLLGPKNSEG
LHSTRDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYIAGLSGSTWYMSTLYSH
PDFPEKGPEEINKELMKNVSHNPLLLLTPQKIKRYVESLWRKKSSGQPVTFTDIFGMLIG
ETLIHNRMNTTLSSLKEKVNTGQCPLPLFTCLHVKPDVSELMFADWVEFSPFEIGMAKYG
TFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSKGSTMEEE
LENITAKHIVSNDSSDSDDESQGPKGTEHEEAEREYQNDNQASWVQRMLMALVSDSALFN
TREGRAGKVHNFMLGLNLNTSYPMSPLRDFTMQESLDEDELDAAVADPDEFEQIYEPLDV
KSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMN
KLPFPKIDPYVFDREGLKECYVFKPKNPDVEKDCPTIIHFVLANINFRKYKAPGVPRETN
EEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMYFNTLNNIDVIKNAIVESIEYRR
QNPSRCSVSLSSVEARRFFNKEFLSKPTA
|
| External Links |
|---|
| GenBank ID Protein
| AAR17479.1 |
| UniProtKB/Swiss-Prot ID
| A4IFJ5 |
| UniProtKB/Swiss-Prot Entry Name
| PA24A_BOVIN |
| PDB IDs
|
|
| GenBank Gene ID
| AY363688 |
| GeneCard ID
| PLA2G4A |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
