| Identification |
|---|
| BMDB Protein ID
| BMDBP02090 |
| Secondary Accession Numbers
| None |
| Name
| Cleavage and polyadenylation specificity factor subunit 5 |
| Synonyms
|
Not Available
|
| Gene Name
| NUDT21 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in hydrolase activity |
| Specific Function
| Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 18 |
| Locus
| Not Available |
| SNPs
| NUDT21 |
| Gene Sequence
|
>684 bp
ATGTCCGTGGTGCCGCCCAATCGCTCGCAGACCGGCTGGCCCCGGGGAGTCACCCAGTTC
GGCAACAAGTATATCCAGCAGACCAAGCCCCTCACCCTGGAGCGCACCATCAACCTGTAC
CCTCTTACCAATTACACTTTTGGTACAAAAGAACCCCTCTATGAGAAGGACAGCTCTGTT
GCAGCCAGATTTCAGCGCATGAGGGAGGAATTTGATAAAATTGGCATGAGGAGGACTGTA
GAAGGGGTTTTGATTGTCCATGAGCACCGGCTACCCCATGTGTTACTGCTACAGCTGGGA
ACAACGTTCTTCAAATTACCTGGTGGTGAGCTTAATCCAGGAGAAGATGAAGTTGAAGGA
CTAAAACGCTTAATGACAGAGATATTGGGTCGTCAAGATGGAGTCCTGCAAGACTGGGTT
ATCGATGACTGCATTGGCAACTGGTGGAGACCAAATTTTGAGCCTCCTCAGTATCCATAT
ATTCCTGCACATATTACAAAACCTAAGGAACATAAGAAGTTGTTTCTGGTTCAGCTTCAA
GAGAAAGCCTTGTTTGCAGTCCCTAAAAATTACAAGCTGGTAGCTGCACCGCTATTTGAA
CTGTATGACAATGCACCTGGATATGGACCCATCATTTCTAGTCTCCCCCAGCTTTTGAGC
AGGTTCAATTTTATATACAACTGA
|
| Protein Properties |
|---|
| Number of Residues
| 227 |
| Molecular Weight
| 26227.0 |
| Theoretical pI
| 9.18 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Cleavage and polyadenylation specificity factor subunit 5
MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSV
AARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEG
LKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQ
EKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN
|
| External Links |
|---|
| GenBank ID Protein
| AAI02698.1 |
| UniProtKB/Swiss-Prot ID
| Q3ZCA2 |
| UniProtKB/Swiss-Prot Entry Name
| CPSF5_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| BC102697 |
| GeneCard ID
| NUDT21 |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
